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Yorodumi- PDB-6wmc: Crystal structure of a soluble variant of full-length human APOBE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6wmc | ||||||
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Title | Crystal structure of a soluble variant of full-length human APOBEC3G (pH 9.0) | ||||||
Components |
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Keywords | HYDROLASE/DNA / APOBEC3G / ANTIVIRAL DEFENSE / HYDROLASE / DNA CYTIDINE DEAMINASE / HYDROLASE-DNA complex | ||||||
Function / homology | DNA Function and homology information | ||||||
Biological species | Homo sapiens (human) Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.49 Å | ||||||
Authors | Maiti, A. / Matsuo, H. | ||||||
Funding support | United States, 1items
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Citation | Journal: J.Mol.Biol. / Year: 2020 Title: Crystal Structure of a Soluble APOBEC3G Variant Suggests ssDNA to Bind in a Channel that Extends between the Two Domains. Authors: Maiti, A. / Myint, W. / Delviks-Frankenberry, K.A. / Hou, S. / Kanai, T. / Balachandran, V. / Sierra Rodriguez, C. / Tripathi, R. / Kurt Yilmaz, N. / Pathak, V.K. / Schiffer, C.A. / Matsuo, H. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6wmc.cif.gz | 103.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6wmc.ent.gz | 65.1 KB | Display | PDB format |
PDBx/mmJSON format | 6wmc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6wmc_validation.pdf.gz | 425.8 KB | Display | wwPDB validaton report |
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Full document | 6wmc_full_validation.pdf.gz | 429.3 KB | Display | |
Data in XML | 6wmc_validation.xml.gz | 15.3 KB | Display | |
Data in CIF | 6wmc_validation.cif.gz | 19.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wm/6wmc ftp://data.pdbj.org/pub/pdb/validation_reports/wm/6wmc | HTTPS FTP |
-Related structure data
Related structure data | 6wmaC 6wmbC 5k81S 6buxS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 42467.715 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: APOBEC3G / Production host: Escherichia coli (E. coli) |
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#2: DNA chain | Mass: 533.406 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) |
#3: Chemical | ChemComp-ZN / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.33 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 9 / Details: 0.1M BICINE (pH 9.0) and 10% w/v PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Feb 8, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.49→50 Å / Num. obs: 5588 / % possible obs: 89 % / Redundancy: 2.8 % / Biso Wilson estimate: 69.24 Å2 / CC1/2: 0.985 / Rmerge(I) obs: 0.158 / Net I/σ(I): 5.08 |
Reflection shell | Resolution: 3.49→3.63 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.497 / Mean I/σ(I) obs: 1.37 / Num. unique obs: 482 / CC1/2: 0.718 / % possible all: 83.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6BUX CHAIN A AND 5K81 CHAIN A Resolution: 3.49→47.72 Å / SU ML: 0.5317 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 28.896
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 66.28 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.49→47.72 Å
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Refine LS restraints |
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LS refinement shell |
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