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Open data
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Basic information
Entry | Database: PDB / ID: 6wce | ||||||
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Title | Structure of the periplasmic binding protein P5PA | ||||||
![]() | Pyridoxal-5-phosphate binding protein A (P5PA) | ||||||
![]() | TRANSPORT PROTEIN / type-II periplasmic binding protein / VitaminB6 / nutrition | ||||||
Function / homology | PYRIDOXAL-5'-PHOSPHATE![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pan, C. / Zimmer, A. / Shah, M. / Huynh, M. / Lai, C.C.L. / Sit, B. / Hooda, Y. / Moraes, T.F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Actinobacillus utilizes a binding protein-dependent ABC transporter to acquire the active form of vitamin B 6 . Authors: Pan, C. / Zimmer, A. / Shah, M. / Huynh, M.S. / Lai, C.C. / Sit, B. / Hooda, Y. / Curran, D.M. / Moraes, T.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 145.6 KB | Display | ![]() |
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PDB format | ![]() | 104.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 765.1 KB | Display | ![]() |
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Full document | ![]() | 766.8 KB | Display | |
Data in XML | ![]() | 15.5 KB | Display | |
Data in CIF | ![]() | 22.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4r73S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39158.699 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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#2: Chemical | ChemComp-PLP / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.85 Å3/Da / Density % sol: 33.54 % |
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Crystal grow | Temperature: 294.15 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 0.05M potassium thiocyanate, 27.5% PEG MME 2000 |
-Data collection
Diffraction | Mean temperature: 105 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 23, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→66.624 Å / Num. obs: 28927 / % possible obs: 92.82 % / Redundancy: 5.1 % / Biso Wilson estimate: 7.94 Å2 / CC1/2: 0.996 / Net I/σ(I): 24.68 |
Reflection shell | Resolution: 1.754→1.817 Å / Num. unique obs: 2856 / CC1/2: 0.985 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4R73 Resolution: 1.754→66.62 Å / SU ML: 0.17 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 18.8515 / Stereochemistry target values: GeoStd + Monomer Library
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 9.54 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.754→66.62 Å
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Refine LS restraints |
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LS refinement shell |
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