+
Open data
-
Basic information
Entry | Database: PDB / ID: 6w7b | ||||||
---|---|---|---|---|---|---|---|
Title | K2P2.1 (TREK-1), 0 mM K+ | ||||||
![]() | Potassium channel subfamily K member 2 | ||||||
![]() | METAL TRANSPORT / ion channel / K2P / TREK1 / TREK-1 | ||||||
Function / homology | ![]() TWIK related potassium channel (TREK) / Phase 4 - resting membrane potential / positive regulation of cellular response to hypoxia / cardiac ventricle development / negative regulation of cardiac muscle cell proliferation / stabilization of membrane potential / potassium ion leak channel activity / astrocyte projection / potassium channel inhibitor activity / negative regulation of DNA biosynthetic process ...TWIK related potassium channel (TREK) / Phase 4 - resting membrane potential / positive regulation of cellular response to hypoxia / cardiac ventricle development / negative regulation of cardiac muscle cell proliferation / stabilization of membrane potential / potassium ion leak channel activity / astrocyte projection / potassium channel inhibitor activity / negative regulation of DNA biosynthetic process / outward rectifier potassium channel activity / cochlea development / voltage-gated potassium channel activity / calyx of Held / response to axon injury / response to mechanical stimulus / axon terminus / potassium ion transmembrane transport / voltage-gated potassium channel complex / regulation of membrane potential / potassium ion transport / memory / cellular response to hypoxia / G protein-coupled receptor signaling pathway / apical plasma membrane / axon / neuronal cell body / cell surface / endoplasmic reticulum / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lolicato, M. / Minor, D.L. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: K 2P channel C-type gating involves asymmetric selectivity filter order-disorder transitions. Authors: Lolicato, M. / Natale, A.M. / Abderemane-Ali, F. / Crottes, D. / Capponi, S. / Duman, R. / Wagner, A. / Rosenberg, J.M. / Grabe, M. / Minor Jr., D.L. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 220.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 178 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 672.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 680.4 KB | Display | |
Data in XML | ![]() | 21.4 KB | Display | |
Data in CIF | ![]() | 28.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6w7cC ![]() 6w7dC ![]() 6w7eC ![]() 6w82C ![]() 6w83C ![]() 6w84C ![]() 6w85C ![]() 6w86C ![]() 6w87C ![]() 6w88C ![]() 6w8aC ![]() 6w8cC ![]() 6w8fC ![]() 6cq6S S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
-Protein / Sugars , 2 types, 3 molecules AB![](data/chem/img/B7G.gif)
![](data/chem/img/B7G.gif)
#1: Protein | Mass: 34303.938 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #5: Sugar | ChemComp-B7G / | |
---|
-Non-polymers , 5 types, 10 molecules ![](data/chem/img/CD.gif)
![](data/chem/img/D12.gif)
![](data/chem/img/OCT.gif)
![](data/chem/img/K.gif)
![](data/chem/img/D10.gif)
![](data/chem/img/D12.gif)
![](data/chem/img/OCT.gif)
![](data/chem/img/K.gif)
![](data/chem/img/D10.gif)
#2: Chemical | #3: Chemical | #4: Chemical | #6: Chemical | #7: Chemical | ChemComp-D10 / | |
---|
-Details
Has ligand of interest | Y |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.75 Å3/Da / Density % sol: 67.23 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 18, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 3.88→45.8 Å / Num. obs: 10002 / % possible obs: 99.3 % / Redundancy: 4.9 % / Rmerge(I) obs: 0.196 / Net I/σ(I): 2.95 |
Reflection shell | Resolution: 3.88→4 Å / Num. unique obs: 2771 / CC1/2: 0.264 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 6CQ6 Resolution: 3.88→14.96 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.899 / Cross valid method: THROUGHOUT / SU Rfree Blow DPI: 0.76
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 248.2 Å2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 1.19 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.88→14.96 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3.88→3.93 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Refine-ID: X-RAY DIFFRACTION
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|