- PDB-6w04: Crystal structure of HAD hydrolase, family IA, variant 3 from Ent... -
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Basic information
Entry
Database: PDB / ID: 6w04
Title
Crystal structure of HAD hydrolase, family IA, variant 3 from Entamoeba histolytica HM-1:IMSS
Components
HAD hydrolase, family IA, variant 3
Keywords
HYDROLASE / SSGCID / Entamoeba histolytica / HAD hydrolase / family IA / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease
Function / homology
Haloacid dehalogenase-like hydrolase / Haloacid dehalogenase-like hydrolase / Phosphoglycolate phosphatase-like, domain 2 / HAD hydrolase, subfamily IA / HAD superfamily / HAD-like superfamily / hydrolase activity / HAD hydrolase, family IA, variant 3
Function and homology information
Biological species
Entamoeba histolytica (eukaryote)
Method
X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.95 Å
Mass: 18.015 Da / Num. of mol.: 151 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
N
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.52 Å3/Da / Density % sol: 51.3 %
Crystal grow
Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 24.5 mg/mL EnhiA.01283.a.AE1.PS38608 (labeled as BrabA.17285.c.AE2) in 3 mM magnesium chloride against MCSG1_A2-C2 optimization screen, condition g3 (200 mM ammonium sulfate, 26% w/v ...Details: 24.5 mg/mL EnhiA.01283.a.AE1.PS38608 (labeled as BrabA.17285.c.AE2) in 3 mM magnesium chloride against MCSG1_A2-C2 optimization screen, condition g3 (200 mM ammonium sulfate, 26% w/v PEG3350, 100 mM Bis-Tris-HCl, pH 5.4), cryoprotectant: 15% ethylene glycol + 3 mM magnesium chloride in two steps, tray: 314701g3, puck wrw2-2, for experimental phasing, a crystal from MCSG1_A2-C2 optimization screen, condition e7 (300 mM lithium sulfate, 24% w/v PEG3350, 100 mM Bis-Tris-HCl, pH 5.0) was soaked for 20 seconds in 90% reservoir + 10% 5 M sodium iodide in ethylene glycol, followed by another 20-second soak in 80% reservoir + 20% 5 M sodium iodide in ethylene glycol, vitrified in liquid nitrogen, tray 314703e7, puck ynn3-8
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