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- PDB-6vow: Crystal structure of multi-copper oxidase from Pseudomonas Thermo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6vow | ||||||||||||
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Title | Crystal structure of multi-copper oxidase from Pseudomonas Thermotolerans | ||||||||||||
![]() | multicopper oxidase | ||||||||||||
![]() | OXIDOREDUCTASE / LACCASE / COPPER OXIDASE / ACOUSTIC DROPLET EJECTION / LIGNIN | ||||||||||||
Function / homology | CU-O-CU LINKAGE / COPPER (II) ION![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Coler, E.A. / Soares, A.S. / Collins, R.E. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of multi-copper oxidase from Pseudomonas Thermotolerans Authors: Coler, E.A. / Soares, A.S. / Collins, R.E. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 190.1 KB | Display | ![]() |
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PDB format | ![]() | 147.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 280.5 KB | Display | ![]() |
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Full document | ![]() | 280.5 KB | Display | |
Data in XML | ![]() | 1.2 KB | Display | |
Data in CIF | ![]() | 5.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4f7kS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50819.754 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-C2O / | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.64 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, sitting drop Details: Crystallized in plastic sitting drop trays, 30 nl of protein (concentration 10 mg/mL) combined with 30 nl precipitant: 0.10 M Ammonium acetate 10 %(w/v) PEG 3350 Solution was equilibrated ...Details: Crystallized in plastic sitting drop trays, 30 nl of protein (concentration 10 mg/mL) combined with 30 nl precipitant: 0.10 M Ammonium acetate 10 %(w/v) PEG 3350 Solution was equilibrated against a reservoir of 60% PEG 3350. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Aug 10, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9202 Å / Relative weight: 1 |
Reflection | Resolution: 1.92→50 Å / Num. obs: 31326 / % possible obs: 90.2 % / Redundancy: 19.36 % / CC1/2: 0.958 / Rmerge(I) obs: 0.42 / Net I/σ(I): 7.17 |
Reflection shell | Resolution: 1.92→1.97 Å / Rmerge(I) obs: 0.502 / Num. unique obs: 1659 / CC1/2: 0.973 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4f7k Resolution: 1.92→30 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.946 / SU B: 11.056 / SU ML: 0.133 / Cross valid method: THROUGHOUT / ESU R Free: 0.159 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.788 Å2
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Refinement step | Cycle: 1 / Resolution: 1.92→30 Å
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Refine LS restraints |
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