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Yorodumi- PDB-6vmw: Crystal structure of the F316A mutant of GoxA soaked with glycine -
+Open data
-Basic information
Entry | Database: PDB / ID: 6vmw | ||||||
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Title | Crystal structure of the F316A mutant of GoxA soaked with glycine | ||||||
Components | Glycine oxidase | ||||||
Keywords | OXIDOREDUCTASE / CTQ | ||||||
Function / homology | L-Lysine epsilon oxidase, N-terminal / L-lysine epsilon oxidase, C-terminal / L-Lysine epsilon oxidase N-terminal / L-lysine epsilon oxidase C-terminal domain / metal ion binding / Uncharacterized protein Function and homology information | ||||||
Biological species | Pseudoalteromonas luteoviolacea DSM 6061 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.99 Å | ||||||
Authors | Yukl, E.T. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2020 Title: Roles of active-site residues in catalysis, substrate binding, cooperativity, and the reaction mechanism of the quinoprotein glycine oxidase. Authors: Mamounis, K.J. / Yukl, E.T. / Davidson, V.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6vmw.cif.gz | 1.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6vmw.ent.gz | 1 MB | Display | PDB format |
PDBx/mmJSON format | 6vmw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6vmw_validation.pdf.gz | 474.8 KB | Display | wwPDB validaton report |
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Full document | 6vmw_full_validation.pdf.gz | 525.7 KB | Display | |
Data in XML | 6vmw_validation.xml.gz | 128.6 KB | Display | |
Data in CIF | 6vmw_validation.cif.gz | 188.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vm/6vmw ftp://data.pdbj.org/pub/pdb/validation_reports/vm/6vmw | HTTPS FTP |
-Related structure data
Related structure data | 6vl7C 6vmfC 6vmvC 3bywS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 91509.758 Da / Num. of mol.: 4 / Mutation: F316A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudoalteromonas luteoviolacea DSM 6061 (bacteria) Gene: N475_19905 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A161XU12 #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-NA / | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.93 % |
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Crystal grow | Temperature: 293 K / Method: batch mode / pH: 5.5 Details: Protein at 10 mg/mL was combined in a 1:1 ratio with precipitant containing 19% PEG 3350, 0.1 M citrate pH 5.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 15, 2019 |
Radiation | Monochromator: Double-crystal, Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.99→47.21 Å / Num. obs: 240912 / % possible obs: 99.1 % / Redundancy: 3.5 % / CC1/2: 0.994 / Rmerge(I) obs: 0.1 / Rpim(I) all: 0.061 / Rrim(I) all: 0.117 / Net I/σ(I): 9 |
Reflection shell | Resolution: 1.99→2.02 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.56 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 11746 / CC1/2: 0.535 / Rpim(I) all: 0.359 / Rrim(I) all: 0.669 / % possible all: 98.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3BYW Resolution: 1.99→45.941 Å / SU ML: 0.25 / Cross valid method: THROUGHOUT / σ(F): 1.91 / Phase error: 21.25 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 168.93 Å2 / Biso mean: 32.2642 Å2 / Biso min: 4.48 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.99→45.941 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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