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- PDB-6val: Cryo-EM structure of an undecameric chicken CALHM1 and human CALH... -

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Basic information

Entry
Database: PDB / ID: 6val
TitleCryo-EM structure of an undecameric chicken CALHM1 and human CALHM2 chimera
ComponentsGreen fluorescent protein, CALHM1,CALMH2 chimera
KeywordsMEMBRANE PROTEIN / taste / assembly / calcium / chimera
Function / homology
Function and homology information


voltage-gated monoatomic ion channel activity / calcium-activated cation channel activity / monoatomic cation channel activity / bioluminescence / generation of precursor metabolites and energy / positive regulation of apoptotic process / membrane / plasma membrane
Similarity search - Function
Calcium homeostasis modulator protein 1 / Calcium homeostasis modulator family / Calcium homeostasis modulator / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein
Similarity search - Domain/homology
Calcium homeostasis modulator 1 / Green fluorescent protein / Calcium homeostasis modulator protein 2
Similarity search - Component
Biological speciesAequorea victoria (jellyfish)
Gallus gallus (chicken)
Homo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.87 Å
AuthorsSyrjanen, J.L. / Chou, T.H. / Furukawa, H.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) United States
National Institutes of Health/National Institute of Mental Health (NIH/NIMH)
CitationJournal: Nat Struct Mol Biol / Year: 2020
Title: Structure and assembly of calcium homeostasis modulator proteins.
Authors: Johanna L Syrjanen / Kevin Michalski / Tsung-Han Chou / Timothy Grant / Shanlin Rao / Noriko Simorowski / Stephen J Tucker / Nikolaus Grigorieff / Hiro Furukawa /
Abstract: The biological membranes of many cell types contain large-pore channels through which a wide variety of ions and metabolites permeate. Examples include connexin, innexin and pannexin, which form gap ...The biological membranes of many cell types contain large-pore channels through which a wide variety of ions and metabolites permeate. Examples include connexin, innexin and pannexin, which form gap junctions and/or bona fide cell surface channels. The most recently identified large-pore channels are the calcium homeostasis modulators (CALHMs), through which ions and ATP permeate in a voltage-dependent manner to control neuronal excitability, taste signaling and pathologies of depression and Alzheimer's disease. Despite such critical biological roles, the structures and patterns of their oligomeric assembly remain unclear. Here, we reveal the structures of two CALHMs, chicken CALHM1 and human CALHM2, by single-particle cryo-electron microscopy (cryo-EM), which show novel assembly of the four transmembrane helices into channels of octamers and undecamers, respectively. Furthermore, molecular dynamics simulations suggest that lipids can favorably assemble into a bilayer within the larger CALHM2 pore, but not within CALHM1, demonstrating the potential correlation between pore size, lipid accommodation and channel activity.
History
DepositionDec 17, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 29, 2020Provider: repository / Type: Initial release
Revision 1.1Feb 12, 2020Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Feb 26, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last

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Structure visualization

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Assembly

Deposited unit
A: Green fluorescent protein, CALHM1,CALMH2 chimera
B: Green fluorescent protein, CALHM1,CALMH2 chimera
C: Green fluorescent protein, CALHM1,CALMH2 chimera
D: Green fluorescent protein, CALHM1,CALMH2 chimera
E: Green fluorescent protein, CALHM1,CALMH2 chimera
F: Green fluorescent protein, CALHM1,CALMH2 chimera
G: Green fluorescent protein, CALHM1,CALMH2 chimera
H: Green fluorescent protein, CALHM1,CALMH2 chimera
I: Green fluorescent protein, CALHM1,CALMH2 chimera
J: Green fluorescent protein, CALHM1,CALMH2 chimera
K: Green fluorescent protein, CALHM1,CALMH2 chimera


Theoretical massNumber of molelcules
Total (without water)759,26011
Polymers759,26011
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area59350 Å2
ΔGint-416 kcal/mol
Surface area134340 Å2

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Components

#1: Protein
Green fluorescent protein, CALHM1,CALMH2 chimera / Protein FAM26B / Calcium homeostasis modulator protein 2


Mass: 69023.617 Da / Num. of mol.: 11
Fragment: GFP + CALHM1 (UNP residues 2-204) + CALMH2 (UNP residues 207-323)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Aequorea victoria (jellyfish), (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Gene: GFP, CALHM1, CALHM2, FAM26B / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: P42212, UniProt: A0A1D5NWS1, UniProt: Q9HA72

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: chimera of chicken CALHM1 and human CALHM2 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Spodoptera frugiperda (fall armyworm)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: unspecified
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 85 % / Chamber temperature: 288.15 K / Details: Blot for 4 sec before plunging

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 57.2 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

EM software
IDNameCategory
1Warpparticle selection
2EPUimage acquisition
13cisTEM3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.87 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 123664 / Symmetry type: POINT

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