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Yorodumi- PDB-6uw0: Engineered variant of I-OnuI meganuclease with improved thermosta... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6uw0 | ||||||
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Title | Engineered variant of I-OnuI meganuclease with improved thermostability and fully altered specificity targeting cholera toxin A subunit | ||||||
Components |
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Keywords | DNA BINDING PROTEIN/DNA / Meganuclease / Homing Endonuclease / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex | ||||||
Function / homology | Homing endonucleases / Endonuclease I-creI / Roll / Alpha Beta / DNA / DNA (> 10) Function and homology information | ||||||
Biological species | synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.72 Å | ||||||
Authors | Werther, R. / Stoddard, B.L. | ||||||
Funding support | United States, 1items
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Citation | Journal: Structure / Year: 2020 Title: Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition. Authors: Lambert, A.R. / Hallinan, J.P. / Werther, R. / Glow, D. / Stoddard, B.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6uw0.cif.gz | 187.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6uw0.ent.gz | 137.7 KB | Display | PDB format |
PDBx/mmJSON format | 6uw0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6uw0_validation.pdf.gz | 265.5 KB | Display | wwPDB validaton report |
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Full document | 6uw0_full_validation.pdf.gz | 265.5 KB | Display | |
Data in XML | 6uw0_validation.xml.gz | 1.4 KB | Display | |
Data in CIF | 6uw0_validation.cif.gz | 7.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uw/6uw0 ftp://data.pdbj.org/pub/pdb/validation_reports/uw/6uw0 | HTTPS FTP |
-Related structure data
Related structure data | 6uvwC 6uwgC 6uwhC 6uwjC 6uwkC 3qqyS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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