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Open data
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Basic information
Entry | Database: PDB / ID: 6ui5 | |||||||||
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Title | Tmn9 in complex with cofactor FAD | |||||||||
![]() | 2-polyprenyl-6-methoxyphenol hydroxylase-like FAD-dependent oxidoreductase | |||||||||
![]() | OXIDOREDUCTASE / Complex / cyclase / UNKNOWN FUNCTION | |||||||||
Function / homology | FAD-binding domain / FAD binding domain / monooxygenase activity / FAD binding / FAD/NAD(P)-binding domain superfamily / FLAVIN-ADENINE DINUCLEOTIDE / 2-polyprenyl-6-methoxyphenol hydroxylase-like FAD-dependent oxidoreductase / Putative FAD-dependent monooxygenase![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Paiva, F.C.R. / Little, R. / Leadlay, P. / Dias, M.V.B. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Tmn9 in complex with cofactor FAD Authors: Paiva, F.C.R. / Little, R. / Leadlay, P. / Dias, M.V.B. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 402.4 KB | Display | ![]() |
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PDB format | ![]() | 330.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 412.1 KB | Display | ![]() |
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Full document | ![]() | 419.3 KB | Display | |
Data in XML | ![]() | 2.8 KB | Display | |
Data in CIF | ![]() | 12.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5xgvS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54334.340 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.87 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop Details: 30% w/v PEG 8000, 100 mM sodium cacodylate pH 6,5, 200 mM sodium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 26, 2018 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.976255 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.26→49.79 Å / Num. obs: 52087 / % possible obs: 99.6 % / Redundancy: 6.3 % / Biso Wilson estimate: 57.47 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.078 / Rpim(I) all: 0.034 / Rrim(I) all: 0.085 / Net I/σ(I): 12 / Num. measured all: 328949 / Scaling rejects: 22 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5XGV Resolution: 2.4→49.788 Å / SU ML: 0.4 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 30.75
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 222.85 Å2 / Biso mean: 83.9249 Å2 / Biso min: 32.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.4→49.788 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Origin x: 35.2767 Å / Origin y: -11.2119 Å / Origin z: 233.1232 Å
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Refinement TLS group |
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