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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 6ucp | ||||||
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タイトル | Zinc finger 2 from the transcription factor Sp1 DNA binding domain | ||||||
![]() | Transcription factor Sp1 | ||||||
![]() | DNA BINDING PROTEIN / zinc finger | ||||||
機能・相同性 | ![]() cellular response to wortmannin / positive regulation of hydrogen sulfide biosynthetic process / Regulation of CDH11 gene transcription / bHLH transcription factor binding / cellular response to zinc ion starvation / positive regulation by host of viral transcription / response to hydroperoxide / positive regulation of vascular endothelial cell proliferation / NFE2L2 regulating tumorigenic genes / histone acetyltransferase binding ...cellular response to wortmannin / positive regulation of hydrogen sulfide biosynthetic process / Regulation of CDH11 gene transcription / bHLH transcription factor binding / cellular response to zinc ion starvation / positive regulation by host of viral transcription / response to hydroperoxide / positive regulation of vascular endothelial cell proliferation / NFE2L2 regulating tumorigenic genes / histone acetyltransferase binding / positive regulation of amyloid-beta formation / RNA polymerase II transcribes snRNA genes / positive regulation of blood vessel endothelial cell migration / cellular response to estrogen stimulus / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / transcription repressor complex / Activation of gene expression by SREBF (SREBP) / transcription coregulator binding / protein-DNA complex / RNA polymerase II transcription regulatory region sequence-specific DNA binding / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / euchromatin / Oncogene Induced Senescence / PPARA activates gene expression / histone deacetylase binding / cellular response to insulin stimulus / positive regulation of angiogenesis / sequence-specific double-stranded DNA binding / rhythmic process / double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / molecular adaptor activity / DNA-binding transcription factor activity, RNA polymerase II-specific / transcription cis-regulatory region binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / positive regulation of gene expression / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus / metal ion binding / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
![]() | Rao, S.R. / Horne, W.S. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Proteomimetic Zinc Finger Domains with Modified Metal-binding beta-Turns. 著者: Rao, S.R. / Horne, W.S. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 125 KB | 表示 | ![]() |
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PDB形式 | ![]() | 100.4 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
関連構造データ | ![]() 6pv0C ![]() 6pv1C ![]() 6pv2C ![]() 6pv3C ![]() 6ucoC C: 同じ文献を引用 ( |
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類似構造データ | |
その他のデータベース |
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リンク
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集合体
登録構造単位 | ![]()
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1 |
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NMR アンサンブル |
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要素
#1: タンパク質・ペプチド | 分子量: 3833.391 Da / 分子数: 1 / 断片: zinc finger 2, residues 654-684 / 由来タイプ: 合成 / 由来: (合成) ![]() |
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#2: 化合物 | ChemComp-ZN / |
研究の焦点であるリガンドがあるか | N |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||
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NMR実験 |
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試料調製
詳細 | タイプ: solution 内容: 1.3 mM zinc finger 2 from transcription factor Sp1 DNA-binding domain, Met-Nle mutant, 10 mM [U-2H] TRIS, 0.05 mM DSS, 1.56 mM zinc chloride, 90% H2O/10% D2O Label: sample_1 / 溶媒系: 90% H2O/10% D2O | ||||||||||||||||||||
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試料 |
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試料状態 | イオン強度: 10 mM / Label: conditions_1 / pH: 6.5 pH* / 圧: 1 atm / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 700 MHz |
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解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 3 | |||||||||||||||
代表構造 | 選択基準: lowest energy | |||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 10 / 登録したコンフォーマーの数: 10 |