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- PDB-6u53: Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6u53 | ||||||||||||
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Title | Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire C (ZC) | ||||||||||||
![]() | Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire C (ZC) | ||||||||||||
![]() | IMMUNE SYSTEM / Antibody / nanobody / Ebola / filovirus | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Taylor, A.B. / Sherwood, L.J. / Hart, P.J. / Hayhurst, A. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Paratope Duality and Gullying are Among the Atypical Recognition Mechanisms Used by a Trio of Nanobodies to Differentiate Ebolavirus Nucleoproteins. Authors: Sherwood, L.J. / Taylor, A.B. / Hart, P.J. / Hayhurst, A. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.4 KB | Display | ![]() |
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PDB format | ![]() | 47.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 421 KB | Display | ![]() |
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Full document | ![]() | 422.9 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Data in CIF | ![]() | 9.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6u50C ![]() 6u51SC ![]() 6u52C ![]() 6u54C ![]() 6u55C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 13376.943 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Semi-synthetic single pot library Nomad 1 based upon Lama glama Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.05 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 1.0 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1M Tris pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Apr 8, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 1.49→31.24 Å / Num. obs: 13542 / % possible obs: 68.3 % / Redundancy: 12.3 % / Biso Wilson estimate: 29.18 Å2 / CC1/2: 1 / Rpim(I) all: 0.012 / Rrim(I) all: 0.043 / Net I/σ(I): 23.3 |
Reflection shell | Resolution: 1.49→1.58 Å / Redundancy: 12.3 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 678 / CC1/2: 0.557 / Rpim(I) all: 0.555 / Rrim(I) all: 1.97 / % possible all: 21.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6U51 Resolution: 1.49→31.24 Å / SU ML: 0.2049 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 38.3306
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.87 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.49→31.24 Å
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Refine LS restraints |
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LS refinement shell |
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