coumarin biosynthetic process / response to molecule of fungal origin / 2-oxoglutarate-dependent dioxygenase activity / L-ascorbic acid binding / metal ion binding 類似検索 - 分子機能
分子量: 36285.492 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: This protein is a truncated version of hyoscyamine 6-hydroxylase from Datura metel (Uniprot ID: Q6EZB3). Missing residues - two flexible loops. Residues SNA at N-terminus - remained after ...詳細: This protein is a truncated version of hyoscyamine 6-hydroxylase from Datura metel (Uniprot ID: Q6EZB3). Missing residues - two flexible loops. Residues SNA at N-terminus - remained after cleavage of a purification tag by TEV protease. 由来: (組換発現) Datura metel (キチガイナスビ) / 遺伝子: H6H / プラスミド: pMCSG19 / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: Q6EZB3
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: Protein sample: 29 mg/ml tH6H in water, 100 mM N-oxalylglycine, 10 mM hyoscyamine. Mother liquor: 100 mM strontium chloride, 200 mM sodium formate, 20% PEG 3350.