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- PDB-6tsl: Marasmius oreades agglutinin (MOA) in complex with the truncated ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6tsl | ||||||
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Title | Marasmius oreades agglutinin (MOA) in complex with the truncated PVPRAHS synthetic substrate | ||||||
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![]() | SUGAR BINDING PROTEIN / fungal chimerolectin / papain-like cysteine protease / protease-substrate complex / calcium-binding protein / manganese-binding protein | ||||||
Function / homology | Agglutinin, C-terminal / Agglutinin C-terminal / Ricin-type beta-trefoil lectin domain-like / Lectin domain of ricin B chain profile. / Ricin B, lectin domain / Ricin B-like lectins / Papain-like cysteine peptidase superfamily / metal ion binding / Agglutinin![]() | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cordara, G. / Manna, D. / Krengel, U. | ||||||
![]() | ![]() Title: Crystal structure of MOA in complex with a peptide fragment: A protease caught in flagranti . Authors: Manna, D. / Cordara, G. / Krengel, U. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.2 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 22 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6tsmC ![]() 6tsnC ![]() 6tsoC ![]() 6tsqC ![]() 6tsrC ![]() 6yh0C ![]() 3ef2S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein / Protein/peptide , 2 types, 2 molecules AAABBB
#1: Protein | Mass: 32296.645 Da / Num. of mol.: 1 / Mutation: C215A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: pT7-LO / Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 468.569 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: synthetic peptide substrate / Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) |
-Sugars , 2 types, 2 molecules
#3: Polysaccharide | alpha-L-fucopyranose-(1-2)-[alpha-D-galactopyranose-(1-3)]beta-D-galactopyranose Source method: isolated from a genetically manipulated source |
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#4: Polysaccharide | alpha-L-fucopyranose-(1-2)-[alpha-D-galactopyranose-(1-3)]alpha-D-galactopyranose Source method: isolated from a genetically manipulated source |
-Non-polymers , 4 types, 214 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Chemical | ChemComp-EDO / | #7: Chemical | #8: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 62.68 % / Description: rod-like crystal |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1 M Na-acetate pH 5.0, 18% PEG 8000, 0.25 M sodium acetate, 10 mM CaCl2, 5 mM DTT |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 30, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97908 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→46.7 Å / Num. obs: 86228 / % possible obs: 99.7 % / Redundancy: 9.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.097 / Rpim(I) all: 0.033 / Rrim(I) all: 0.103 / Net I/σ(I): 10.7 |
Reflection shell | Resolution: 1.4→1.42 Å / Redundancy: 9.3 % / Rmerge(I) obs: 7.367 / Mean I/σ(I) obs: 0.3 / Num. unique obs: 4078 / CC1/2: 0.367 / Rrim(I) all: 8.292 / Rsym value: 2.668 / % possible all: 93.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3EF2 Resolution: 1.4→46.7 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.973 / SU B: 2.091 / SU ML: 0.068 / Cross valid method: FREE R-VALUE / ESU R: 0.052 / ESU R Free: 0.053 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.914 Å2
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Refinement step | Cycle: LAST / Resolution: 1.4→46.7 Å
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Refine LS restraints |
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LS refinement shell |
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