AA13 Lytic polysaccharide monooxygenase from Aspergillus oryzae partially in Cu(II) state
要素
AoAA13
キーワード
OXIDOREDUCTASE / beta-sandwich fold / low dose
機能・相同性
Cellulose/chitin-binding protein, N-terminal / Lytic polysaccharide mono-oxygenase, cellulose-degrading / membrane / metal ion binding / COPPER (II) ION / Inactive AA13 family lytic polysaccharide monooxygenase
温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5 詳細: 0.1 M buffer system (II) (0.2 M L-malic acid, 0.04 M MES, 0.4 M Tris) pH 5, 0.2 M Zn-acetate, 23% PEG 3000. The crystals were soaked in 0.09 M Buffer system (II) pH 5, 22.5% PEG 3000, 0.5 M ...詳細: 0.1 M buffer system (II) (0.2 M L-malic acid, 0.04 M MES, 0.4 M Tris) pH 5, 0.2 M Zn-acetate, 23% PEG 3000. The crystals were soaked in 0.09 M Buffer system (II) pH 5, 22.5% PEG 3000, 0.5 M Cu-acetate, 1 mg/ml BSA Temp details: Room temperature