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Yorodumi- PDB-6sxe: Crystal Structure of the Voltage-Gated Sodium Channel NavMs (F208... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6sxe | ||||||
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Title | Crystal Structure of the Voltage-Gated Sodium Channel NavMs (F208L) in complex with Endoxifen (2.6 Angstrom resolution) | ||||||
Components | Ion transport protein | ||||||
Keywords | METAL TRANSPORT / Voltage Gated Sodium Channel / Membrane protein | ||||||
Function / homology | Function and homology information voltage-gated sodium channel complex / membrane depolarization during action potential / voltage-gated sodium channel activity Similarity search - Function | ||||||
Biological species | Magnetococcus marinus MC-1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Sula, A. / Hollingworth, D. / Wallace, B.A. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Mol.Cell / Year: 2021 Title: A tamoxifen receptor within a voltage-gated sodium channel. Authors: Sula, A. / Hollingworth, D. / Ng, L.C.T. / Larmore, M. / DeCaen, P.G. / Wallace, B.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6sxe.cif.gz | 131.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6sxe.ent.gz | 102.2 KB | Display | PDB format |
PDBx/mmJSON format | 6sxe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6sxe_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 6sxe_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 6sxe_validation.xml.gz | 13.6 KB | Display | |
Data in CIF | 6sxe_validation.cif.gz | 17.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sx/6sxe ftp://data.pdbj.org/pub/pdb/validation_reports/sx/6sxe | HTTPS FTP |
-Related structure data
Related structure data | 6sx5C 6sx7C 6sxcC 6sxfC 6sxgC 6z8cC 5hvxS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 31185.730 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Magnetococcus marinus MC-1 (bacteria) / Gene: Mmc1_0798 / Production host: Escherichia coli (E. coli) / References: UniProt: A0L5S6 |
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-Non-polymers , 5 types, 58 molecules
#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-7PG / | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.22 Å3/Da / Density % sol: 76.43 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 0.1 M MgCl2 hexahydrate, 0.1 M HEPES buffer, pH 8, 30% v/v polyethylene glycol (PEG) 500 and 4% ethylene glycol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X13 / Wavelength: 0.97966 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 2, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97966 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→52.54 Å / Num. obs: 20152 / % possible obs: 99.1 % / Redundancy: 13.1 % / CC1/2: 0.999 / Net I/σ(I): 14.6 |
Reflection shell | Resolution: 2.6→2.72 Å / Redundancy: 13.8 % / Mean I/σ(I) obs: 1.6 / Num. unique obs: 2435 / CC1/2: 0.826 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5HVX Resolution: 2.6→52.54 Å / Cor.coef. Fo:Fc: 0.875 / Cor.coef. Fo:Fc free: 0.87 / SU R Cruickshank DPI: 0.285 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.286 / SU Rfree Blow DPI: 0.243 / SU Rfree Cruickshank DPI: 0.245
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Displacement parameters | Biso max: 276.3 Å2 / Biso mean: 108.44 Å2 / Biso min: 45.82 Å2
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Refine analyze | Luzzati coordinate error obs: 0.39 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.6→52.54 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.62 Å / Rfactor Rfree error: 0 / Total num. of bins used: 46
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Refinement TLS params. | Method: refined / Origin x: 17.2493 Å / Origin y: 6.0926 Å / Origin z: 32.0674 Å
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Refinement TLS group | Selection details: { A|* } |