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- PDB-6st1: Taurine ABC transporter substrate binding protein TauA from E. co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6st1 | ||||||
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Title | Taurine ABC transporter substrate binding protein TauA from E. coli in complex with 2-(N-Morpholino)ethanesulfonic acid (MES) | ||||||
![]() | Taurine-binding periplasmic protein | ||||||
![]() | TRANSPORT PROTEIN / taurine substrate binding protein / ABC transporter | ||||||
Function / homology | ![]() cellular response to sulfur starvation / alkanesulfonate transmembrane transport / cellular response to sulfate starvation / taurine transmembrane transport / transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Beis, K. / Qu, F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Desolvation of the substrate-binding protein TauA dictates ligand specificity for the alkanesulfonate ABC importer TauABC. Authors: Qu, F. / ElOmari, K. / Wagner, A. / De Simone, A. / Beis, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 135.4 KB | Display | ![]() |
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PDB format | ![]() | 103.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 458 KB | Display | ![]() |
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Full document | ![]() | 459.6 KB | Display | |
Data in XML | ![]() | 26.7 KB | Display | |
Data in CIF | ![]() | 39.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ssyC ![]() 6st0C ![]() 6stlSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32010.322 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Met1 is from cloning Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: tauA, ssiA, yaiR, b0365, JW0357 / Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-IOD / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.92 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.25 M sodium iodide, 0.1 M Bis-Tris propane, 24% (w/v) PEG 3350 + 10 mM MES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 17, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9686 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→40.97 Å / Num. obs: 77044 / % possible obs: 97 % / Redundancy: 4.7 % / CC1/2: 1 / Rmerge(I) obs: 0.09 / Net I/σ(I): 8 |
Reflection shell | Resolution: 1.55→1.59 Å / Rmerge(I) obs: 1.22 / Mean I/σ(I) obs: 1 / Num. unique obs: 5553 / CC1/2: 0.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6STL Resolution: 1.55→40.97 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.55→40.97 Å
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