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- PDB-6sei: Recognition and processing of branched DNA substrates by Slx1-Slx... -

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Basic information

Entry
Database: PDB / ID: 6sei
TitleRecognition and processing of branched DNA substrates by Slx1-Slx4 nuclease
Components
  • (Structure-specific endonuclease subunit ...) x 2
  • DNA (32-MER)
KeywordsDNA BINDING PROTEIN / Resolvase / Structure-Selective endonuclease / Endonuclease
Function / homology
Function and homology information


Slx1-Slx4 complex / crossover junction DNA endonuclease activity / 5'-flap endonuclease activity / double-strand break repair via homologous recombination / DNA recombination / DNA replication / DNA repair / regulation of DNA-templated transcription / DNA binding / metal ion binding
Similarity search - Function
Structure-specific endonuclease subunit Slx4, ascomycetes / : / Structure-specific endonuclease subunit SLX1, C-terminal / Structure-specific endonuclease subunit Slx1 / : / Structure-specific endonuclease subunit Slx4 / Slx4 endonuclease / HMG-I/HMG-Y, DNA-binding, conserved site / HMG-I and HMG-Y DNA-binding domain (A+T-hook). / GIY-YIG endonuclease ...Structure-specific endonuclease subunit Slx4, ascomycetes / : / Structure-specific endonuclease subunit SLX1, C-terminal / Structure-specific endonuclease subunit Slx1 / : / Structure-specific endonuclease subunit Slx4 / Slx4 endonuclease / HMG-I/HMG-Y, DNA-binding, conserved site / HMG-I and HMG-Y DNA-binding domain (A+T-hook). / GIY-YIG endonuclease / GIY-YIG endonuclease superfamily / GIY-YIG catalytic domain / GIY-YIG domain profile. / DNA binding domain with preference for A/T rich regions / AT hook, DNA-binding motif / Zinc finger, RING/FYVE/PHD-type
Similarity search - Domain/homology
DNA / DNA (> 10) / Structure-specific endonuclease subunit SLX4 / GIY-YIG domain-containing protein / Structure-specific endonuclease subunit SLX4
Similarity search - Component
Biological speciesThielavia terrestris (fungus)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.69 Å
AuthorsGaur, V. / Zajko, W. / Nirwal, S. / Szlachcic, A. / Gapinska, M. / Nowotny, M.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Wellcome Trust098022 United Kingdom
CitationJournal: Nucleic Acids Res. / Year: 2019
Title: Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease.
Authors: Gaur, V. / Ziajko, W. / Nirwal, S. / Szlachcic, A. / Gapinska, M. / Nowotny, M.
History
DepositionJul 30, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 25, 2019Provider: repository / Type: Initial release
Revision 1.1Oct 16, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed ..._citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Oct 23, 2019Group: Data collection / Database references / Structure summary
Category: audit_author / citation_author / Item: _audit_author.name / _citation_author.name
Revision 1.3Dec 18, 2019Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.name
Revision 1.4Jan 24, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr2_symmetry / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Structure-specific endonuclease subunit SLX1
B: Structure-specific endonuclease subunit SLX4
C: Structure-specific endonuclease subunit SLX1
D: Structure-specific endonuclease subunit SLX4
E: DNA (32-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)105,62711
Polymers105,2855
Non-polymers3426
Water4,972276
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration, Slx1 and Slx4 form a stable complex in gel filtration experiments.
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8470 Å2
ΔGint-63 kcal/mol
Surface area39160 Å2
MethodPISA
Unit cell
Length a, b, c (Å)60.075, 92.700, 93.251
Angle α, β, γ (deg.)90.000, 91.603, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Structure-specific endonuclease subunit ... , 2 types, 4 molecules ACBD

#1: Protein Structure-specific endonuclease subunit SLX1


Mass: 35744.246 Da / Num. of mol.: 2 / Mutation: E79Q
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thielavia terrestris (fungus) / Gene: SLX1, THITE_2107775 / Production host: Escherichia coli (E. coli)
References: UniProt: G2QV68, Hydrolases; Acting on ester bonds
#2: Protein Structure-specific endonuclease subunit SLX4


Mass: 11833.439 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thielavia terrestris (fungus) / Gene: SLX4, TT172_LOCUS87 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A3S4CYR8, UniProt: G2RCY5*PLUS

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DNA chain , 1 types, 1 molecules E

#3: DNA chain DNA (32-MER)


Mass: 10129.532 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Random sequence / Source: (synth.) synthetic construct (others)

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Non-polymers , 3 types, 282 molecules

#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#5: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 276 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.88 Å3/Da / Density % sol: 57.35 %
Crystal growTemperature: 298.15 K / Method: vapor diffusion, sitting drop / pH: 6
Details: 0.1 M MES (pH 6.0), 0.2 M calcium chloride dihydrate, and 20% (v/v) PEG 6000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.9184 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 3, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9184 Å / Relative weight: 1
ReflectionResolution: 2.69→46.61 Å / Num. obs: 28286 / % possible obs: 98.9 % / Redundancy: 3.49 % / CC1/2: 0.985 / Net I/σ(I): 7.23
Reflection shellResolution: 2.69→2.85 Å / Redundancy: 3.59 % / Mean I/σ(I) obs: 2.44 / Num. unique obs: 4554 / CC1/2: 0.82 / % possible all: 98.8

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6SEH

6seh


Resolution: 2.69→46.61 Å / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 29.0784
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2655 1451 5.13 %
Rwork0.2084 26835 -
obs0.2152 28286 98.93 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 38.56 Å2
Refinement stepCycle: LAST / Resolution: 2.69→46.61 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5596 647 6 276 6525
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00376482
X-RAY DIFFRACTIONf_angle_d0.63738934
X-RAY DIFFRACTIONf_chiral_restr0.0409970
X-RAY DIFFRACTIONf_plane_restr0.00451035
X-RAY DIFFRACTIONf_dihedral_angle_d14.07014290
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.69-2.790.34751400.27322653X-RAY DIFFRACTION93.98
2.79-2.90.33991430.2542720X-RAY DIFFRACTION94.48
2.9-3.030.31631410.23692669X-RAY DIFFRACTION94.51
3.03-3.190.30231400.2332672X-RAY DIFFRACTION93.56
3.19-3.390.30381400.22772648X-RAY DIFFRACTION93.67
3.39-3.650.27831420.21372694X-RAY DIFFRACTION94.36
3.65-4.020.21241410.19642696X-RAY DIFFRACTION94.2
4.02-4.60.25321420.18642689X-RAY DIFFRACTION93.79
4.6-5.790.22151420.18032691X-RAY DIFFRACTION94.19
5.79-46.360.24141440.20942734X-RAY DIFFRACTION93.53
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.18145726168-0.362431460758-0.593340647770.3656118943220.9454112154713.39534094419-0.00344758596058-0.0403915293902-0.1130265671260.0752292863276-0.09183838855410.1341830348110.144260469429-0.2576398678970.1282596500930.434831646969-0.04177843045970.05661110777870.135835585212-0.01481786440770.0859778063738-52.5832400919-40.96370912572.91225096001
24.21219897444-0.0362969855304-0.2459545055661.972129749561.594362972125.472369884670.0787290620895-0.777292729517-0.7493324761851.08328530057-0.1713388924860.08267174700431.09638706242-0.06828442429330.1423583503710.591060663634-0.01576638641520.02541074399410.4864934095210.04277234465630.359560455955-63.84502343-40.42305944389.00861346776
30.7470186340560.136117596999-0.6052407551280.9787346539-0.003797068609162.063427206660.0216592146908-0.189996671655-0.0261502337440.180135533807-0.1236134560360.1635873551990.1252386700880.09444996322590.131723929640.379125903391-0.03655979741540.02350216050760.163120276417-0.02990415062350.0922444822971-45.7215167771-42.76215891965.07882906668
42.15488196954-1.80986177846-1.49330585911.973932687651.136879604342.2181343668-0.1183560488810.296790382179-0.2628040392580.11938514227-0.166773849970.1796310762920.435261660254-0.2828095912910.1312308391210.753733180188-0.09920982523820.1313508374390.3767434557560.01413305526710.197353818061-42.3875423957-45.471283427717.1323222419
50.215812302573-0.06366230984160.09381466799330.2055695822790.1382432986120.753089401322-0.100594159875-0.02855860050670.06638416744710.0591030831301-0.007287891327190.00220345890242-0.0904403721332-0.0327365284499-0.2100004310940.481566725535-0.0225196374444-0.01035221498540.142990302893-0.0519769164670.0798780589032-39.7786747781-32.78377049777.37472670215
61.68311702820.0299874921587-0.6995715037741.675822260210.5094665265171.85713661102-0.09296324754320.195390472890.208201261271-0.0372140368449-0.002225334693750.0326705315194-0.2275350797810.07206732644550.1353394596870.492960090051-0.01072910546380.02867437184020.1980548196020.01617327925380.0770250520079-41.3316437073-28.0478785632-0.329156140562
71.3384183091-0.331219290986-0.9067644005840.3260121168940.5698343744611.159860697160.05010408428320.322643116583-0.0236597509814-0.0400711958456-0.09859938723810.0733723114055-0.0509938493356-0.2147873873430.04524116494510.4583965880970.007602969697570.001481204186990.205012335316-0.009215775861270.105188086615-34.5409112632-46.7739385501-20.7191899607
81.32013462828-0.129935988885-0.8314359779481.34602575657-0.1051805002432.031464811010.02844400342250.08312928839170.124554998880.00201565428183-0.0495861901577-0.0449735481944-0.3167948914760.0658462250733-0.05545847846720.482552687937-0.02318482118870.02211260731920.1076942299680.003252729811570.0828811806857-24.5231320785-42.6297252698-13.084979683
91.07584703921-0.157118885375-0.6343374598960.380715752627-0.3593876038451.963351618660.01800671129390.266904769542-0.0657357211307-0.2141179198560.01041449578240.0642902393656-0.0390686440047-0.2909054445410.1053465881480.480827789954-0.0156761304175-0.03143506917580.205794140344-0.03205122654910.0585247441416-39.2825780249-47.9464877591-16.3241629332
103.86028075638-0.272833054976-1.051571146171.236836697191.122785800031.18956293172-0.079997105262-0.7750873530110.4162042633230.3910017450410.0342330873261-0.320527787639-0.5398966642050.5396356900580.002286179240860.642814652821-0.0388306720664-0.1146322840310.30275241043-0.03342192580990.191219290991-16.6168851925-54.23190672638.28519069214
113.22281950231-0.8955987202952.839679815640.5291950124640.03624932967234.92996894878-0.02228511014420.0119467177682-0.120676748185-0.1123058236940.03012609533440.04798897813480.482964746381-0.315448247315-0.06502666822640.432856830029-0.0535767675887-0.001470553336780.1393551564620.01858219711730.0897797089619-26.8527899735-60.9006285952-12.7073335871
120.446879383552-0.2197256902280.2479908941840.225450780824-0.1217793274230.512679237488-0.0290914940347-0.0697759157574-0.05731432922130.08549338447670.03916193564560.01220564898020.1487374365280.001362580144230.02832458179380.4382017465420.009922966673490.0087439055930.1541274234030.01958823454690.0665023327098-27.0820301543-60.1221143193-0.388722965307
130.7865094454390.0342533909197-0.6736597221910.05505303491720.09772960484680.881470819088-0.0873145292006-0.279615557535-0.1790111489220.02730406226440.08602266898380.01425702897210.3173637503750.0216012335398-0.000697186450010.5694618057490.01969173812690.003559912845840.2265025656150.04890680669470.116647638425-26.6126289563-57.53276588297.78989171853
142.031435154111.115431264270.8086841745431.644122214252.111030375643.02463141396-0.2281441586650.07125610455450.309168198733-0.166874826203-0.1281553178080.362835143601-0.368773732532-0.3684604989040.340728510340.5058783741910.0425274556771-0.01747992219590.1541632986450.004545050319290.143231459207-56.76413736521.0604734018140.4858829393
150.413203865830.1447776694920.400839935430.1584234578860.1320972686281.341504776280.00366224016850.07283961649350.000499372140789-0.0626407954497-0.01049651741390.0175825578347-0.04166028674110.0812367752284-0.01268671539450.4131758448240.0306660346123-0.003043663824190.145734745386-0.005795168710650.0691460719919-46.75900445242.9321098281140.6724328849
160.4936133208020.1448089945210.04751660494550.4249710085640.5220797995331.28526592073-0.02751884629350.07238315280030.0445387480704-0.2110785511120.0192183377940.013789484733-0.1698127769020.03229700692480.06857227097790.487162959725-0.03093816174550.0001262075876990.137818325162-0.01732516793320.0618129841684-40.74552255650.085889874934435.2213780127
170.5262217042030.2426411746470.9155899326141.994442097781.210961248481.99315152420.0761401538384-0.220456488232-0.08183682205260.0945178020122-0.1369840467590.02043961257660.239312075642-0.0109134316608-0.01290137034320.472448371148-0.0254291529130.0296211829250.1836068885640.0194855852870.0894465487977-44.0995966857-10.462070465344.457806286
183.134030692271.960548971961.523599243232.878876028790.3836278968933.628929867850.0516926601525-0.467855225499-0.03922308629620.129344789006-0.2118418540760.08222229282310.170702301917-0.5012112029110.01447203605940.48151566004-0.002738295747230.03379498105780.310833579390.0500705210880.10746274973-41.38070502564.7091509202568.9580447725
191.15370633076-0.0993645753620.6725072989741.143870753430.4632568281862.696072598260.0792238704668-0.1446330869990.006888648967990.125841291178-0.05448237276580.01513232752220.207413913540.166496545122-0.04342089847970.471066815332-0.03049706667670.0001582650500790.168704803718-0.005158599526290.119427401242-27.03851423026.3863940066864.4713985341
201.226247016950.6433610006190.488371086671.31081800974-0.2866360768833.251404098950.031780087924-0.1582150100290.1012723790270.1527201682340.001421177990270.117616401132-0.0103126618164-0.3559753201850.01457170072430.5134218354190.01106556562360.02510355786080.190611509856-0.003851204850430.115879581347-40.81005831587.896538343361.6920444196
213.702930842440.1163565216070.9142929986390.9974763181280.7145393335431.36429017602-0.0669811479340.582964722421-0.410164433816-0.2392422920730.0678674999651-0.2953528740010.2618700432350.327820040787-0.09918527621620.6819490573260.03957960763210.1485567102750.246300849456-0.0425051881930.23544374544-17.667545460814.209202486638.6334082511
220.9808274015570.222265590979-1.228851221260.0745478934667-0.1541587486372.176079660510.0207204638665-0.0004051644997840.03635152199810.0730784237637-0.01418130549540.0237956608846-0.20254214664-0.024223796417-0.08776937000790.467058933578-0.0157252737386-0.01427318692920.09930486106020.01153960803720.0957591635031-28.03513771620.785402447358.9536447806
231.129931568240.538060250219-0.4882769639940.850744181878-0.2733841807580.717328498837-0.1001429382270.11326164270.066905761756-0.082171772060.06204732136780.069398776682-0.169161565843-0.0917070027235-0.01257318635040.4137174566930.01340148667080.0001234296295460.1669587693150.02185453215230.0913792500858-27.934255167120.254035229647.1303009395
240.779752829640.2414800838840.4305276106510.09552359897410.2705592569141.13553891948-0.1874453779820.1756004876670.177211891925-0.1824655244790.131643298675-0.000657970213625-0.489042843707-0.06087424198470.01727924367880.583939911737-0.0966390004274-0.008968001925310.201323201927-0.003184140642840.129588481779-27.881626103217.543072646138.7416587728
253.954063164760.819992337028-0.5454072049873.238615753610.3971524468593.14560423401-0.178336542387-0.609154353013-1.160779564870.536338733605-0.6867775804350.1855297450470.8457640999380.1266944007110.7111047098911.15034211959-0.04972911717430.1247522558270.6099474634560.09008719809020.79421552128-58.2374725532-27.94358466426.7577984043
265.38859202086-3.369897244582.275018423432.818456729430.06692461261414.07777485458-0.06686494591130.8559081952810.660993218108-0.619922194271-0.518757362513-0.110902485736-0.09138642941880.2689054894210.5858743474420.898237072610.0730615686055-0.07530781221610.5216283082870.1449037423570.453469010661-58.1000708426-6.3428586643218.498244456
271.030202302790.152992203494-0.3952190903590.4560590501781.332645472084.70623249684-0.219594895664-0.0760744835898-0.3314874743780.264262111835-0.153042018931-0.159388352690.4535697514440.08185882382660.3337502840490.974765421043-0.09749100473050.3288483463070.6516313071880.1440819896780.938689811599-61.6318099373-30.724392049525.9029855925
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 41 )
2X-RAY DIFFRACTION2chain 'A' and (resid 42 through 59 )
3X-RAY DIFFRACTION3chain 'A' and (resid 60 through 83 )
4X-RAY DIFFRACTION4chain 'A' and (resid 84 through 115 )
5X-RAY DIFFRACTION5chain 'A' and (resid 116 through 156 )
6X-RAY DIFFRACTION6chain 'A' and (resid 157 through 198 )
7X-RAY DIFFRACTION7chain 'A' and (resid 199 through 245 )
8X-RAY DIFFRACTION8chain 'A' and (resid 246 through 285 )
9X-RAY DIFFRACTION9chain 'A' and (resid 286 through 312 )
10X-RAY DIFFRACTION10chain 'B' and (resid 842 through 861 )
11X-RAY DIFFRACTION11chain 'B' and (resid 862 through 872 )
12X-RAY DIFFRACTION12chain 'B' and (resid 873 through 899 )
13X-RAY DIFFRACTION13chain 'B' and (resid 900 through 925 )
14X-RAY DIFFRACTION14chain 'C' and (resid 4 through 59 )
15X-RAY DIFFRACTION15chain 'C' and (resid 60 through 83 )
16X-RAY DIFFRACTION16chain 'C' and (resid 84 through 143 )
17X-RAY DIFFRACTION17chain 'C' and (resid 144 through 207 )
18X-RAY DIFFRACTION18chain 'C' and (resid 208 through 222 )
19X-RAY DIFFRACTION19chain 'C' and (resid 223 through 285 )
20X-RAY DIFFRACTION20chain 'C' and (resid 286 through 311 )
21X-RAY DIFFRACTION21chain 'D' and (resid 842 through 860 )
22X-RAY DIFFRACTION22chain 'D' and (resid 861 through 872 )
23X-RAY DIFFRACTION23chain 'D' and (resid 873 through 900 )
24X-RAY DIFFRACTION24chain 'D' and (resid 901 through 925 )
25X-RAY DIFFRACTION25chain 'E' and (resid 2 through 11 )
26X-RAY DIFFRACTION26chain 'E' and (resid 12 through 26 )
27X-RAY DIFFRACTION27chain 'E' and (resid 27 through 33 )

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