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- PDB-6s3j: Crystal Structure of lipase from Geobacillus stearothermophilus T... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6s3j | ||||||
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Title | Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant E134C/F149C | ||||||
![]() | Lipase | ||||||
![]() | HYDROLASE / lipase / methanol / organic solvent / disulfide bond | ||||||
Function / homology | triacylglycerol lipase / triacylglycerol lipase activity / lipid catabolic process / Alpha/Beta hydrolase fold / extracellular region / metal ion binding / triacylglycerol lipase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gihaz, S. / Bash, Y. / Rush, I. / Shahar, A. / Pazy, Y. / Fishman, A. | ||||||
![]() | ![]() Title: Bridges to Stability: Engineering Disulfide Bonds Towards Enhanced Lipase Biodiesel Synthesis Authors: Gihaz, S. / Bash, Y. / Rush, I. / Shahar, A. / Pazy, Y. / Fishman, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 177.2 KB | Display | ![]() |
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PDB format | ![]() | 138.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 417.3 KB | Display | ![]() |
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Full document | ![]() | 419.5 KB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 28.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6s3gC ![]() 6s3vC ![]() 4x6uS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43863.000 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-CA / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 / Details: 0.2M sodium citrate, 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 6, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→44.56 Å / Num. obs: 32174 / % possible obs: 98.2 % / Redundancy: 4.3 % / Biso Wilson estimate: 18.35 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.092 / Rrim(I) all: 0.104 / Net I/σ(I): 17.5 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.755 / Mean I/σ(I) obs: 4.2 / Num. unique obs: 4652 / CC1/2: 0.768 / Rrim(I) all: 0.84 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4X6U Resolution: 1.9→44.56 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 18.2 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→44.56 Å
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