温度: 277 K / 手法: 蒸発脱水法 詳細: protein concentration of 14 mg/mL in 0.1M HEPES pH 7.5, 10% w/v polyethylene glycol 8,000, 8% v/v ethylene glycol. Crystals of the complex of RNase AS with GMP were prepared by soaking ...詳細: protein concentration of 14 mg/mL in 0.1M HEPES pH 7.5, 10% w/v polyethylene glycol 8,000, 8% v/v ethylene glycol. Crystals of the complex of RNase AS with GMP were prepared by soaking crystals of native protein in a solution containing GMP at 20 mM.
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.54 Å / 相対比: 1
反射
解像度: 2→30 Å / Num. obs: 23972 / % possible obs: 99.9 % / 冗長度: 6.9 % / Net I/σ(I): 20.2
反射 シェル
解像度: 2→2.03 Å / Num. unique obs: 1170
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.5.0110
精密化
HKL-2000
データ削減
HKL-2000
データスケーリング
PHASER
位相決定
精密化
構造決定の手法: 分子置換 / 解像度: 2→30 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.919 / SU B: 4.463 / SU ML: 0.123 / 交差検証法: THROUGHOUT / ESU R: 0.209 / ESU R Free: 0.188 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS