+Open data
-Basic information
Entry | Database: PDB / ID: 6r5f | ||||||
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Title | Crystal structure of RIP1 kinase in complex with DHP77 | ||||||
Components | Receptor-interacting serine/threonine-protein kinase 1 | ||||||
Keywords | TRANSFERASE / Inhibitor / Complex | ||||||
Function / homology | Function and homology information regulation of ATP:ADP antiporter activity / ripoptosome assembly / positive regulation of interleukin-6-mediated signaling pathway / positive regulation of miRNA processing / ripoptosome assembly involved in necroptotic process / death domain binding / ripoptosome / Defective RIPK1-mediated regulated necrosis / TRIF-mediated programmed cell death / TLR3-mediated TICAM1-dependent programmed cell death ...regulation of ATP:ADP antiporter activity / ripoptosome assembly / positive regulation of interleukin-6-mediated signaling pathway / positive regulation of miRNA processing / ripoptosome assembly involved in necroptotic process / death domain binding / ripoptosome / Defective RIPK1-mediated regulated necrosis / TRIF-mediated programmed cell death / TLR3-mediated TICAM1-dependent programmed cell death / Microbial modulation of RIPK1-mediated regulated necrosis / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / programmed necrotic cell death / TNF signaling / Caspase activation via Death Receptors in the presence of ligand / T cell apoptotic process / positive regulation of macrophage differentiation / SARS-CoV-1-mediated effects on programmed cell death / necroptotic signaling pathway / JUN kinase kinase kinase activity / peptidyl-serine autophosphorylation / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / positive regulation of necroptotic process / death-inducing signaling complex / RIP-mediated NFkB activation via ZBP1 / negative regulation of necroptotic process / positive regulation of tumor necrosis factor-mediated signaling pathway / death receptor binding / TRP channels / positive regulation of extrinsic apoptotic signaling pathway / positive regulation of programmed cell death / positive regulation of programmed necrotic cell death / TNFR1-induced proapoptotic signaling / RIPK1-mediated regulated necrosis / positive regulation of execution phase of apoptosis / necroptotic process / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / response to tumor necrosis factor / signaling adaptor activity / negative regulation of canonical NF-kappaB signal transduction / extrinsic apoptotic signaling pathway / tumor necrosis factor-mediated signaling pathway / TICAM1, RIP1-mediated IKK complex recruitment / IKK complex recruitment mediated by RIP1 / TNFR1-induced NF-kappa-B signaling pathway / positive regulation of interleukin-8 production / negative regulation of extrinsic apoptotic signaling pathway / Regulation of TNFR1 signaling / positive regulation of JNK cascade / protein catabolic process / Regulation of necroptotic cell death / cellular response to hydrogen peroxide / cellular response to growth factor stimulus / positive regulation of inflammatory response / positive regulation of non-canonical NF-kappaB signal transduction / positive regulation of neuron apoptotic process / positive regulation of reactive oxygen species metabolic process / Ovarian tumor domain proteases / positive regulation of tumor necrosis factor production / cellular response to tumor necrosis factor / positive regulation of NF-kappaB transcription factor activity / positive regulation of canonical NF-kappaB signal transduction / response to oxidative stress / amyloid fibril formation / Potential therapeutics for SARS / protein autophosphorylation / receptor complex / endosome membrane / non-specific serine/threonine protein kinase / Ub-specific processing proteases / protein kinase activity / intracellular signal transduction / inflammatory response / positive regulation of protein phosphorylation / positive regulation of apoptotic process / protein serine kinase activity / protein serine/threonine kinase activity / apoptotic process / ubiquitin protein ligase binding / protein-containing complex binding / positive regulation of gene expression / negative regulation of apoptotic process / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / protein-containing complex / mitochondrion / ATP binding / identical protein binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.25 Å | ||||||
Authors | Thorpe, J.H. / Campobasso, N. / Harris, P.A. | ||||||
Citation | Journal: J.Med.Chem. / Year: 2019 Title: Discovery and Lead-Optimization of 4,5-Dihydropyrazoles as Mono-Kinase Selective, Orally Bioavailable and Efficacious Inhibitors of Receptor Interacting Protein 1 (RIP1) Kinase. Authors: Harris, P.A. / Faucher, N. / George, N. / Eidam, P.M. / King, B.W. / White, G.V. / Anderson, N.A. / Bandyopadhyay, D. / Beal, A.M. / Beneton, V. / Berger, S.B. / Campobasso, N. / Campos, S. ...Authors: Harris, P.A. / Faucher, N. / George, N. / Eidam, P.M. / King, B.W. / White, G.V. / Anderson, N.A. / Bandyopadhyay, D. / Beal, A.M. / Beneton, V. / Berger, S.B. / Campobasso, N. / Campos, S. / Capriotti, C.A. / Cox, J.A. / Daugan, A. / Donche, F. / Fouchet, M.H. / Finger, J.N. / Geddes, B. / Gough, P.J. / Grondin, P. / Hoffman, B.L. / Hoffman, S.J. / Hutchinson, S.E. / Jeong, J.U. / Jigorel, E. / Lamoureux, P. / Leister, L.K. / Lich, J.D. / Mahajan, M.K. / Meslamani, J. / Mosley, J.E. / Nagilla, R. / Nassau, P.M. / Ng, S.L. / Ouellette, M.T. / Pasikanti, K.K. / Potvain, F. / Reilly, M.A. / Rivera, E.J. / Sautet, S. / Schaeffer, M.C. / Sehon, C.A. / Sun, H. / Thorpe, J.H. / Totoritis, R.D. / Ward, P. / Wellaway, N. / Wisnoski, D.D. / Woolven, J.M. / Bertin, J. / Marquis, R.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6r5f.cif.gz | 214.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6r5f.ent.gz | 171.1 KB | Display | PDB format |
PDBx/mmJSON format | 6r5f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r5/6r5f ftp://data.pdbj.org/pub/pdb/validation_reports/r5/6r5f | HTTPS FTP |
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-Related structure data
Related structure data | 6ocqC 4itjS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: Protein | Mass: 34596.891 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RIPK1, RIP, RIP1 / Production host: Baculovirus expression vector pFastBac1-HM References: UniProt: Q13546, non-specific serine/threonine protein kinase #2: Chemical | ChemComp-JSW / [( |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.28 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.2M Ammonium Fluoride, 20% PEG3350 and 4mM DHP77 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 9, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 3.25→69.46 Å / Num. obs: 18831 / % possible obs: 99.1 % / Redundancy: 3.3 % / Biso Wilson estimate: 88.89 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.137 / Rpim(I) all: 0.089 / Rrim(I) all: 0.164 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 3.25→3.98 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.453 / Num. unique obs: 8493 / CC1/2: 0.772 / Rpim(I) all: 0.297 / Rrim(I) all: 0.543 / % possible all: 98.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4ITJ Resolution: 3.25→69.46 Å / Cor.coef. Fo:Fc: 0.864 / Cor.coef. Fo:Fc free: 0.795 / Rfactor Rfree error: 0 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.595
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Displacement parameters | Biso mean: 85.7 Å2
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Refine analyze | Luzzati coordinate error obs: 0.56 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 3.25→69.46 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.25→3.45 Å / Rfactor Rfree error: 0 / Total num. of bins used: 9
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