PDB-6ql7: Structure of fatty acid synthase complex with bound gamma subunit...

Basic information

• Entry: Database: PDB / ID: 6ql7
• Title: Structure of fatty acid synthase complex with bound gamma subunit from Saccharomyces cerevisiae at 4.6 angstrom

Components

• Fatty acid synthase subunit alpha
• Fatty acid synthase subunit beta
• Translation machinery-associated protein 17

• Keywords: TRANSFERASE / Fatty acid synthase / Acyl carrier protein / Ketosynthase / Ketoreductase / Enoyl reductase / Dehydratase / Malonyl/palmitoyl transferase / Acetyl transferase / Phosphopantetheine transferase

Function / homology

Function:

fatty-acyl-CoA synthase system / fatty acid synthase complex / fatty-acyl-CoA synthase activity / palmitoyltransferase activity / [acyl-carrier-protein] S-acetyltransferase / proteasome regulatory particle assembly / [acyl-carrier-protein] S-acetyltransferase activity / holo-[acyl-carrier-protein] synthase activity / oleoyl-[acyl-carrier-protein] hydrolase / fatty acyl-[ACP] hydrolase activity / [acyl-carrier-protein] S-malonyltransferase / 3-hydroxyacyl-[acyl-carrier-protein] dehydratase / enoyl-[acyl-carrier-protein] reductase (NADPH) activity / [acyl-carrier-protein] S-malonyltransferase activity / beta-ketoacyl-[acyl-carrier-protein] synthase I / (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity / 3-oxoacyl-[acyl-carrier-protein] reductase / 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity / enoyl-[acyl-carrier-protein] reductase (NADH) / long-chain fatty acid biosynthetic process / enoyl-[acyl-carrier-protein] reductase (NADH) activity / fatty acid synthase activity / 3-oxoacyl-[acyl-carrier-protein] synthase activity / lipid droplet / fatty acid biosynthetic process / protein-macromolecule adaptor activity / magnesium ion binding / mitochondrion / nucleus / cytosol / cytoplasm

Domain/homology:

Translation machinery-associated protein 17 / Fatty acid synthase beta subunit AflB /Fas1-like, fungi / Fatty acid synthase, meander beta sheet domain / Fatty acid synthase subunit beta, N-terminal domain / N-terminal domain in fatty acid synthase subunit beta / Fatty acid synthase meander beta sheet domain / Fatty acid synthase subunit beta, insertion domain / Fatty acid synthase beta subunit AflB /Fas1-like, central domain / Fatty acid synthase alpha subunit, yeast / Fatty acid synthase subunit beta/Fas1-like, helical / Fatty acid synthase subunit alpha, acyl carrier domain / Fatty acid synthase subunit alpha Acyl carrier domain / Fatty acid synthase type I, helical / : / Fatty acid synthase type I helical domain / : / Fatty acid synthase / N-terminal of MaoC-like dehydratase / FAS1-like, dehydratase domain region / Starter unit:ACP transacylase / Starter unit:ACP transacylase in aflatoxin biosynthesis / Holo-[acyl carrier protein] synthase / Phosphopantetheine-protein transferase domain / MaoC-like dehydratase domain / MaoC like domain / 4'-phosphopantetheinyl transferase domain / 4'-phosphopantetheinyl transferase domain superfamily / 4'-phosphopantetheinyl transferase superfamily / HotDog domain superfamily / Acyl transferase / Acyl transferase domain / Acyl transferase domain in polyketide synthase (PKS) enzymes. / Acyl transferase domain superfamily / Acyl transferase/acyl hydrolase/lysophospholipase / Beta-ketoacyl synthase, active site / Ketosynthase family 3 (KS3) active site signature. / Ketosynthase family 3 (KS3) domain profile. / Beta-ketoacyl synthase, N-terminal / Beta-ketoacyl synthase, C-terminal / Polyketide synthase, beta-ketoacyl synthase domain / Beta-ketoacyl synthase, N-terminal domain / Beta-ketoacyl synthase, C-terminal domain / Thiolase-like / Phosphopantetheine attachment site. / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Aldolase-type TIM barrel / NAD(P)-binding domain superfamily

Component:

Fatty acid synthase subunit beta / Fatty acid synthase subunit alpha / Translation machinery-associated protein 17

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.6 Å
• Authors: Singh, K. / Graf, B. / Linden, A. / Sautner, V. / Urlaub, H. / Tittmann, K. / Stark, H. / Chari, A.

Funding support

Germany, 1items

Organization	Grant number	Country
German Research Foundation	SFB860-TP A5	Germany

• Citation: Journal: Cell / Year: 2020; Title: Discovery of a Regulatory Subunit of the Yeast Fatty Acid Synthase.; Authors: Kashish Singh / Benjamin Graf / Andreas Linden / Viktor Sautner / Henning Urlaub / Kai Tittmann / Holger Stark / Ashwin Chari / ; Abstract: Fatty acid synthases (FASs) are central to metabolism but are also of biotechnological interest for the production of fine chemicals and biofuels from renewable resources. During fatty acid synthesis, the growing fatty acid chain is thought to be shuttled by the dynamic acyl carrier protein domain to several enzyme active sites. Here, we report the discovery of a γ subunit of the 2.6 megadalton α-βS. cerevisiae FAS, which is shown by high-resolution structures to stabilize a rotated FAS conformation and rearrange ACP domains from equatorial to axial positions. The γ subunit spans the length of the FAS inner cavity, impeding reductase activities of FAS, regulating NADPH turnover by kinetic hysteresis at the ketoreductase, and suppressing off-pathway reactions at the enoylreductase. The γ subunit delineates the functional compartment within FAS. As a scaffold, it may be exploited to incorporate natural and designed enzymatic activities that are not present in natural FAS.

History

Deposition	Jan 31, 2019	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Mar 18, 2020	Provider: repository / Type: Initial release
Revision 1.1	Apr 8, 2020	Group: Database references / Category: citation Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2	May 15, 2024	Group: Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / struct_ncs_dom_lim / struct_sheet Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id / _struct_sheet.number_strands

Assembly

Deposited unit

A: Fatty acid synthase subunit alpha

B: Fatty acid synthase subunit alpha

C: Fatty acid synthase subunit alpha

D: Fatty acid synthase subunit alpha

E: Fatty acid synthase subunit alpha

F: Fatty acid synthase subunit alpha

G: Fatty acid synthase subunit beta

H: Fatty acid synthase subunit beta

I: Fatty acid synthase subunit beta

J: Fatty acid synthase subunit beta

K: Fatty acid synthase subunit beta

L: Fatty acid synthase subunit beta

M: Translation machinery-associated protein 17

N: Translation machinery-associated protein 17

O: Translation machinery-associated protein 17

P: Translation machinery-associated protein 17

Q: Translation machinery-associated protein 17

R: Translation machinery-associated protein 17

a: Fatty acid synthase subunit alpha

b: Fatty acid synthase subunit alpha

c: Fatty acid synthase subunit alpha

d: Fatty acid synthase subunit alpha

e: Fatty acid synthase subunit alpha

f: Fatty acid synthase subunit alpha

g: Fatty acid synthase subunit beta

h: Fatty acid synthase subunit beta

i: Fatty acid synthase subunit beta

j: Fatty acid synthase subunit beta

k: Fatty acid synthase subunit beta

l: Fatty acid synthase subunit beta

m: Translation machinery-associated protein 17

n: Translation machinery-associated protein 17

o: Translation machinery-associated protein 17

p: Translation machinery-associated protein 17

q: Translation machinery-associated protein 17

r: Translation machinery-associated protein 17

Summary:

• 5.44 MDa, 36 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	5,435,007	36
Polymers	5,435,007	36
Non-polymers	0	0
Water	0	0

1

A: Fatty acid synthase subunit alpha

B: Fatty acid synthase subunit alpha

C: Fatty acid synthase subunit alpha

D: Fatty acid synthase subunit alpha

E: Fatty acid synthase subunit alpha

F: Fatty acid synthase subunit alpha

G: Fatty acid synthase subunit beta

H: Fatty acid synthase subunit beta

I: Fatty acid synthase subunit beta

J: Fatty acid synthase subunit beta

K: Fatty acid synthase subunit beta

L: Fatty acid synthase subunit beta

M: Translation machinery-associated protein 17

N: Translation machinery-associated protein 17

O: Translation machinery-associated protein 17

P: Translation machinery-associated protein 17

Q: Translation machinery-associated protein 17

R: Translation machinery-associated protein 17

Summary:

• defined by author
• 2.72 MDa, 18 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	2,717,504	18
Polymers	2,717,504	18
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

a: Fatty acid synthase subunit alpha

b: Fatty acid synthase subunit alpha

c: Fatty acid synthase subunit alpha

d: Fatty acid synthase subunit alpha

e: Fatty acid synthase subunit alpha

f: Fatty acid synthase subunit alpha

g: Fatty acid synthase subunit beta

h: Fatty acid synthase subunit beta

i: Fatty acid synthase subunit beta

j: Fatty acid synthase subunit beta

k: Fatty acid synthase subunit beta

l: Fatty acid synthase subunit beta

m: Translation machinery-associated protein 17

n: Translation machinery-associated protein 17

o: Translation machinery-associated protein 17

p: Translation machinery-associated protein 17

q: Translation machinery-associated protein 17

r: Translation machinery-associated protein 17

Summary:

• defined by author
• 2.72 MDa, 18 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	2,717,504	18
Polymers	2,717,504	18
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	234.922, 430.307, 422.610
Angle α, β, γ (deg.)	90.000, 97.010, 90.000
Int Tables number	4
Space group name H-M	P1211

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	A
2	1	B
1	2	A
2	2	C
1	3	A
2	3	D
1	4	A
2	4	E
1	5	A
2	5	F
1	6	A
2	6	a
1	7	A
2	7	b
1	8	A
2	8	c
1	9	A
2	9	d
1	10	A
2	10	e
1	11	A
2	11	f
1	12	B
2	12	C
1	13	B
2	13	D
1	14	B
2	14	E
1	15	B
2	15	F
1	16	B
2	16	a
1	17	B
2	17	b
1	18	B
2	18	c
1	19	B
2	19	d
1	20	B
2	20	e
1	21	B
2	21	f
1	22	C
2	22	D
1	23	C
2	23	E
1	24	C
2	24	F
1	25	C
2	25	a
1	26	C
2	26	b
1	27	C
2	27	c
1	28	C
2	28	d
1	29	C
2	29	e
1	30	C
2	30	f
1	31	D
2	31	E
1	32	D
2	32	F
1	33	D
2	33	a
1	34	D
2	34	b
1	35	D
2	35	c
1	36	D
2	36	d
1	37	D
2	37	e
1	38	D
2	38	f
1	39	E
2	39	F
1	40	E
2	40	a
1	41	E
2	41	b
1	42	E
2	42	c
1	43	E
2	43	d
1	44	E
2	44	e
1	45	E
2	45	f
1	46	F
2	46	a
1	47	F
2	47	b
1	48	F
2	48	c
1	49	F
2	49	d
1	50	F
2	50	e
1	51	F
2	51	f
1	52	G
2	52	H
1	53	G
2	53	I
1	54	G
2	54	J
1	55	G
2	55	K
1	56	G
2	56	L
1	57	G
2	57	g
1	58	G
2	58	h
1	59	G
2	59	i
1	60	G
2	60	j
1	61	G
2	61	k
1	62	G
2	62	l
1	63	H
2	63	I
1	64	H
2	64	J
1	65	H
2	65	K
1	66	H
2	66	L
1	67	H
2	67	g
1	68	H
2	68	h
1	69	H
2	69	i
1	70	H
2	70	j
1	71	H
2	71	k
1	72	H
2	72	l
1	73	I
2	73	J
1	74	I
2	74	K
1	75	I
2	75	L
1	76	I
2	76	g
1	77	I
2	77	h
1	78	I
2	78	i
1	79	I
2	79	j
1	80	I
2	80	k
1	81	I
2	81	l
1	82	J
2	82	K
1	83	J
2	83	L
1	84	J
2	84	g
1	85	J
2	85	h
1	86	J
2	86	i
1	87	J
2	87	j
1	88	J
2	88	k
1	89	J
2	89	l
1	90	K
2	90	L
1	91	K
2	91	g
1	92	K
2	92	h
1	93	K
2	93	i
1	94	K
2	94	j
1	95	K
2	95	k
1	96	K
2	96	l
1	97	L
2	97	g
1	98	L
2	98	h
1	99	L
2	99	i
1	100	L
2	100	j
1	101	L
2	101	k
1	102	L
2	102	l
1	103	M
2	103	N
1	104	M
2	104	O
1	105	M
2	105	P
1	106	M
2	106	Q
1	107	M
2	107	R
1	108	M
2	108	m
1	109	M
2	109	n
1	110	M
2	110	o
1	111	M
2	111	p
1	112	M
2	112	q
1	113	M
2	113	r
1	114	N
2	114	O
1	115	N
2	115	P
1	116	N
2	116	Q
1	117	N
2	117	R
1	118	N
2	118	m
1	119	N
2	119	n
1	120	N
2	120	o
1	121	N
2	121	p
1	122	N
2	122	q
1	123	N
2	123	r
1	124	O
2	124	P
1	125	O
2	125	Q
1	126	O
2	126	R
1	127	O
2	127	m
1	128	O
2	128	n
1	129	O
2	129	o
1	130	O
2	130	p
1	131	O
2	131	q
1	132	O
2	132	r
1	133	P
2	133	Q
1	134	P
2	134	R
1	135	P
2	135	m
1	136	P
2	136	n
1	137	P
2	137	o
1	138	P
2	138	p
1	139	P
2	139	q
1	140	P
2	140	r
1	141	Q
2	141	R
1	142	Q
2	142	m
1	143	Q
2	143	n
1	144	Q
2	144	o
1	145	Q
2	145	p
1	146	Q
2	146	q
1	147	Q
2	147	r
1	148	R
2	148	m
1	149	R
2	149	n
1	150	R
2	150	o
1	151	R
2	151	p
1	152	R
2	152	q
1	153	R
2	153	r
1	154	a
2	154	b
1	155	a
2	155	c
1	156	a
2	156	d
1	157	a
2	157	e
1	158	a
2	158	f
1	159	b
2	159	c
1	160	b
2	160	d
1	161	b
2	161	e
1	162	b
2	162	f
1	163	c
2	163	d
1	164	c
2	164	e
1	165	c
2	165	f
1	166	d
2	166	e
1	167	d
2	167	f
1	168	e
2	168	f
1	169	g
2	169	h
1	170	g
2	170	i
1	171	g
2	171	j
1	172	g
2	172	k
1	173	g
2	173	l
1	174	h
2	174	i
1	175	h
2	175	j
1	176	h
2	176	k
1	177	h
2	177	l
1	178	i
2	178	j
1	179	i
2	179	k
1	180	i
2	180	l
1	181	j
2	181	k
1	182	j
2	182	l
1	183	k
2	183	l
1	184	m
2	184	n
1	185	m
2	185	o
1	186	m
2	186	p
1	187	m
2	187	q
1	188	m
2	188	r
1	189	n
2	189	o
1	190	n
2	190	p
1	191	n
2	191	q
1	192	n
2	192	r
1	193	o
2	193	p
1	194	o
2	194	q
1	195	o
2	195	r
1	196	p
2	196	q
1	197	p
2	197	r
1	198	q
2	198	r

NCS domain segments:

Component-ID: _ / Refine code: _

Dom-ID	Ens-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	1	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
1	2	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	2	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
1	3	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	3	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
1	4	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	4	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
1	5	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	5	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
1	6	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	6	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
1	7	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	7	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	8	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	8	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	9	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	9	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	10	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	10	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	11	MET	MET	LYS	LYS	A	A	1 - 1886	1 - 1886
2	11	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	12	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	12	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
1	13	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	13	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
1	14	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	14	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
1	15	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	15	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
1	16	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	16	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
1	17	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	17	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	18	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	18	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	19	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	19	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	20	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	20	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	21	MET	MET	LYS	LYS	B	B	1 - 1886	1 - 1886
2	21	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	22	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	22	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
1	23	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	23	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
1	24	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	24	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
1	25	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	25	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
1	26	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	26	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	27	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	27	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	28	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	28	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	29	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	29	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	30	MET	MET	LYS	LYS	C	C	1 - 1886	1 - 1886
2	30	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	31	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	31	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
1	32	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	32	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
1	33	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	33	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
1	34	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	34	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	35	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	35	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	36	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	36	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	37	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	37	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	38	MET	MET	LYS	LYS	D	D	1 - 1886	1 - 1886
2	38	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	39	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	39	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
1	40	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	40	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
1	41	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	41	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	42	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	42	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	43	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	43	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	44	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	44	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	45	MET	MET	LYS	LYS	E	E	1 - 1886	1 - 1886
2	45	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	46	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
2	46	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
1	47	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
2	47	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	48	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
2	48	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	49	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
2	49	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	50	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
2	50	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	51	MET	MET	LYS	LYS	F	F	1 - 1886	1 - 1886
2	51	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	52	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	52	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
1	53	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	53	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
1	54	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	54	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
1	55	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	55	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
1	56	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	56	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
1	57	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	57	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
1	58	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	58	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	59	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	59	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	60	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	60	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	61	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	61	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	62	SER	SER	GLN	GLN	G	G	5 - 2050	5 - 2050
2	62	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	63	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	63	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
1	64	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	64	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
1	65	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	65	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
1	66	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	66	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
1	67	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	67	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
1	68	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	68	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	69	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	69	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	70	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	70	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	71	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	71	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	72	SER	SER	GLN	GLN	H	H	5 - 2050	5 - 2050
2	72	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	73	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	73	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
1	74	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	74	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
1	75	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	75	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
1	76	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	76	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
1	77	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	77	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	78	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	78	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	79	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	79	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	80	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	80	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	81	SER	SER	GLN	GLN	I	I	5 - 2050	5 - 2050
2	81	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	82	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	82	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
1	83	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	83	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
1	84	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	84	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
1	85	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	85	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	86	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	86	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	87	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	87	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	88	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	88	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	89	SER	SER	GLN	GLN	J	J	5 - 2050	5 - 2050
2	89	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	90	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	90	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
1	91	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	91	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
1	92	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	92	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	93	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	93	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	94	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	94	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	95	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	95	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	96	SER	SER	GLN	GLN	K	K	5 - 2050	5 - 2050
2	96	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	97	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
2	97	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
1	98	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
2	98	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	99	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
2	99	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	100	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
2	100	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	101	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
2	101	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	102	SER	SER	GLN	GLN	L	L	5 - 2050	5 - 2050
2	102	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	103	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	103	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
1	104	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	104	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
1	105	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	105	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
1	106	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	106	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
1	107	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	107	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
1	108	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	108	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
1	109	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	109	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	110	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	110	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	111	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	111	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	112	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	112	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	113	SER	SER	ILE	ILE	M	M	3 - 150	3 - 150
2	113	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	114	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	114	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
1	115	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	115	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
1	116	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	116	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
1	117	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	117	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
1	118	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	118	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
1	119	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	119	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	120	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	120	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	121	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	121	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	122	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	122	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	123	SER	SER	ILE	ILE	N	N	3 - 150	3 - 150
2	123	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	124	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	124	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
1	125	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	125	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
1	126	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	126	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
1	127	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	127	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
1	128	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	128	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	129	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	129	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	130	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	130	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	131	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	131	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	132	SER	SER	ILE	ILE	O	O	3 - 150	3 - 150
2	132	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	133	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	133	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
1	134	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	134	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
1	135	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	135	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
1	136	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	136	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	137	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	137	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	138	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	138	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	139	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	139	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	140	SER	SER	ILE	ILE	P	P	3 - 150	3 - 150
2	140	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	141	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	141	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
1	142	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	142	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
1	143	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	143	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	144	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	144	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	145	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	145	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	146	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	146	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	147	SER	SER	ILE	ILE	Q	Q	3 - 150	3 - 150
2	147	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	148	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
2	148	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
1	149	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
2	149	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	150	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
2	150	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	151	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
2	151	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	152	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
2	152	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	153	SER	SER	ILE	ILE	R	R	3 - 150	3 - 150
2	153	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	154	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
2	154	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
1	155	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
2	155	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	156	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
2	156	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	157	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
2	157	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	158	MET	MET	LYS	LYS	a	S	1 - 1886	1 - 1886
2	158	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	159	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
2	159	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
1	160	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
2	160	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	161	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
2	161	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	162	MET	MET	LYS	LYS	b	T	1 - 1886	1 - 1886
2	162	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	163	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
2	163	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
1	164	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
2	164	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	165	MET	MET	LYS	LYS	c	U	1 - 1886	1 - 1886
2	165	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	166	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
2	166	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
1	167	MET	MET	LYS	LYS	d	V	1 - 1886	1 - 1886
2	167	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	168	MET	MET	LYS	LYS	e	W	1 - 1886	1 - 1886
2	168	MET	MET	LYS	LYS	f	X	1 - 1886	1 - 1886
1	169	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
2	169	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
1	170	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
2	170	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	171	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
2	171	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	172	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
2	172	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	173	SER	SER	GLN	GLN	g	Y	5 - 2050	5 - 2050
2	173	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	174	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
2	174	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
1	175	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
2	175	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	176	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
2	176	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	177	SER	SER	GLN	GLN	h	Z	5 - 2050	5 - 2050
2	177	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	178	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
2	178	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
1	179	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
2	179	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	180	SER	SER	GLN	GLN	i	AA	5 - 2050	5 - 2050
2	180	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	181	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
2	181	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
1	182	SER	SER	GLN	GLN	j	BA	5 - 2050	5 - 2050
2	182	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	183	SER	SER	GLN	GLN	k	CA	5 - 2050	5 - 2050
2	183	SER	SER	GLN	GLN	l	DA	5 - 2050	5 - 2050
1	184	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
2	184	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
1	185	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
2	185	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	186	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
2	186	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	187	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
2	187	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	188	SER	SER	ILE	ILE	m	EA	3 - 150	3 - 150
2	188	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	189	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
2	189	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
1	190	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
2	190	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	191	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
2	191	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	192	SER	SER	ILE	ILE	n	FA	3 - 150	3 - 150
2	192	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	193	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
2	193	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
1	194	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
2	194	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	195	SER	SER	ILE	ILE	o	GA	3 - 150	3 - 150
2	195	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	196	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
2	196	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
1	197	SER	SER	ILE	ILE	p	HA	3 - 150	3 - 150
2	197	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150
1	198	SER	SER	ILE	ILE	q	IA	3 - 150	3 - 150
2	198	SER	SER	ILE	ILE	r	JA	3 - 150	3 - 150

NCS ensembles :

ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198

Components

#1: Protein

A B C D E F a b c d e f
Fatty acid synthase subunit alpha

Details:

Mass: 207184.422 Da / Num. of mol.: 12 / Source method: isolated from a natural source
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
References: UniProt: P19097, fatty-acyl-CoA synthase system, 3-oxoacyl-[acyl-carrier-protein] reductase, beta-ketoacyl-[acyl-carrier-protein] synthase I

#2: Protein

G H I J K L g h i j k l
Fatty acid synthase subunit beta

Details:

Mass: 228940.375 Da / Num. of mol.: 12 / Source method: isolated from a natural source
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
References: UniProt: P07149, fatty-acyl-CoA synthase system, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, enoyl-[acyl-carrier-protein] reductase (NADH), [acyl-carrier-protein] S-acetyltransferase, [acyl-carrier-protein] S-malonyltransferase, oleoyl-[acyl-carrier-protein] hydrolase

#3: Protein

M N O P Q R m n o p q r
Translation machinery-associated protein 17 / ATPase-dedicated chaperone of 17 kDa / ADC17

Details:

Mass: 16792.455 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: TMA17, YDL110C, D2320 / Production host: Escherichia coli (E. coli) / References: UniProt: Q12513

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.9 ų/Da / Density % sol: 68.45 %
• Crystal grow: Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.2 ; Details: 0.1 M MES pH 6.2, 0.15-0.3 M ammonium sulphate, 7-8% (w/v) PEG 8000

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 0.976 Å
• Detector: Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 6, 2018 / Details: CRL Transfocator
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.976 Å / Relative weight: 1
• Reflection: Resolution: 4.6→192.5 Å / Num. obs: 458021 / % possible obs: 99.51 % / Redundancy: 7.2 % / CC_1/2: 0.998 / R_pim(I) all: 0.08786 / Net I/σ(I): 7.95
• Reflection shell: Resolution: 4.6→4.7 Å / Redundancy: 7.4 % / Mean I/σ(I) obs: 0.87 / Num. unique obs: 45686 / CC_1/2: 0.364 / R_pim(I) all: 0.8292 / % possible all: 99.71

Processing

Software

Name	Version	Classification
REFMAC	5.8.0238	refinement
XDS		data reduction
Aimless	0.7.3	data scaling
MOLREP	11.6.04	phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 4.6→192.5 Å / Cor.coef. F_o:F_c: 0.902 / Cor.coef. F_o:F_c free: 0.879 / SU B: 149.24 / SU ML: 1.568 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 1.334
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

	Rfactor	Num. reflection	% reflection	Selection details
Rfree	0.3098	22715	5 %	RANDOM
Rwork	0.2516	-	-	-
obs	0.2545	435383	99.6 %	-

• Solvent computation: Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å

Displacement parameters

B_iso max: 891.56 Ų / B_iso mean: 242.41 Ų / B_iso min: 22.15 Ų

	Baniso -1	Baniso -2	Baniso -3
1-	3.08 Å2	0 Å2	0.08 Å2
2-	-	6.1 Å2	-0 Å2
3-	-	-	-8.93 Å2

Refinement step

Cycle: final / Resolution: 4.6→192.5 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	231252	0	0	0	231252
Num. residues	-	-	-	-	46908

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.003	0.013	374951
X-RAY DIFFRACTION	r_bond_other_d	0.001	0.017	350450
X-RAY DIFFRACTION	r_angle_refined_deg	1.226	1.642	507729
X-RAY DIFFRACTION	r_angle_other_deg	1.126	1.575	816028
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	7.245	5	46775
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	29.679	23.549	18360
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	12.947	15	66132
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	15.027	15	1728
X-RAY DIFFRACTION	r_chiral_restr	0.045	0.2	49560
X-RAY DIFFRACTION	r_gen_planes_refined	0.003	0.02	416705
X-RAY DIFFRACTION	r_gen_planes_other	0.001	0.02	74495

Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-ID	Dom-ID	Auth asym-ID	Number	Rms dev position (Å)
1	1	A	54928	0.01
1	2	B	54928	0.01
2	1	A	54949	0.01
2	2	C	54949	0.01
3	1	A	54943	0.01
3	2	D	54943	0.01
4	1	A	54947	0.01
4	2	E	54947	0.01
5	1	A	54935	0.02
5	2	F	54935	0.02
6	1	A	54882	0.02
6	2	a	54882	0.02
7	1	A	54929	0.01
7	2	b	54929	0.01
8	1	A	54899	0.02
8	2	c	54899	0.02
9	1	A	54893	0.02
9	2	d	54893	0.02
10	1	A	54918	0.02
10	2	e	54918	0.02
11	1	A	54895	0.02
11	2	f	54895	0.02
12	1	B	54940	0.01
12	2	C	54940	0.01
13	1	B	54934	0.02
13	2	D	54934	0.02
14	1	B	54932	0.02
14	2	E	54932	0.02
15	1	B	54936	0.02
15	2	F	54936	0.02
16	1	B	54886	0.02
16	2	a	54886	0.02
17	1	B	54916	0.02
17	2	b	54916	0.02
18	1	B	54915	0.02
18	2	c	54915	0.02
19	1	B	54893	0.02
19	2	d	54893	0.02
20	1	B	54905	0.02
20	2	e	54905	0.02
21	1	B	54899	0.02
21	2	f	54899	0.02
22	1	C	54948	0.01
22	2	D	54948	0.01
23	1	C	54945	0.01
23	2	E	54945	0.01
24	1	C	54946	0.02
24	2	F	54946	0.02
25	1	C	54893	0.02
25	2	a	54893	0.02
26	1	C	54924	0.02
26	2	b	54924	0.02
27	1	C	54916	0.02
27	2	c	54916	0.02
28	1	C	54911	0.02
28	2	d	54911	0.02
29	1	C	54929	0.02
29	2	e	54929	0.02
30	1	C	54914	0.02
30	2	f	54914	0.02
31	1	D	54940	0.01
31	2	E	54940	0.01
32	1	D	54950	0.01
32	2	F	54950	0.01
33	1	D	54879	0.02
33	2	a	54879	0.02
34	1	D	54944	0.02
34	2	b	54944	0.02
35	1	D	54905	0.02
35	2	c	54905	0.02
36	1	D	54900	0.02
36	2	d	54900	0.02
37	1	D	54913	0.02
37	2	e	54913	0.02
38	1	D	54914	0.02
38	2	f	54914	0.02
39	1	E	54932	0.01
39	2	F	54932	0.01
40	1	E	54884	0.02
40	2	a	54884	0.02
41	1	E	54923	0.01
41	2	b	54923	0.01
42	1	E	54908	0.02
42	2	c	54908	0.02
43	1	E	54898	0.02
43	2	d	54898	0.02
44	1	E	54919	0.02
44	2	e	54919	0.02
45	1	E	54905	0.02
45	2	f	54905	0.02
46	1	F	54871	0.02
46	2	a	54871	0.02
47	1	F	54929	0.02
47	2	b	54929	0.02
48	1	F	54908	0.02
48	2	c	54908	0.02
49	1	F	54896	0.02
49	2	d	54896	0.02
50	1	F	54920	0.02
50	2	e	54920	0.02
51	1	F	54897	0.02
51	2	f	54897	0.02
52	1	G	63482	0
52	2	H	63482	0
53	1	G	63470	0.01
53	2	I	63470	0.01
54	1	G	63492	0
54	2	J	63492	0
55	1	G	63487	0
55	2	K	63487	0
56	1	G	63487	0
56	2	L	63487	0
57	1	G	63485	0
57	2	g	63485	0
58	1	G	63487	0
58	2	h	63487	0
59	1	G	63484	0
59	2	i	63484	0
60	1	G	63492	0
60	2	j	63492	0
61	1	G	63484	0
61	2	k	63484	0
62	1	G	63480	0
62	2	l	63480	0
63	1	H	63474	0.01
63	2	I	63474	0.01
64	1	H	63485	0
64	2	J	63485	0
65	1	H	63481	0
65	2	K	63481	0
66	1	H	63486	0
66	2	L	63486	0
67	1	H	63494	0
67	2	g	63494	0
68	1	H	63488	0
68	2	h	63488	0
69	1	H	63496	0
69	2	i	63496	0
70	1	H	63496	0
70	2	j	63496	0
71	1	H	63477	0
71	2	k	63477	0
72	1	H	63480	0
72	2	l	63480	0
73	1	I	63480	0.01
73	2	J	63480	0.01
74	1	I	63470	0.01
74	2	K	63470	0.01
75	1	I	63473	0.01
75	2	L	63473	0.01
76	1	I	63490	0.01
76	2	g	63490	0.01
77	1	I	63468	0.01
77	2	h	63468	0.01
78	1	I	63479	0.01
78	2	i	63479	0.01
79	1	I	63487	0.01
79	2	j	63487	0.01
80	1	I	63473	0.01
80	2	k	63473	0.01
81	1	I	63469	0.01
81	2	l	63469	0.01
82	1	J	63494	0
82	2	K	63494	0
83	1	J	63495	0
83	2	L	63495	0
84	1	J	63501	0
84	2	g	63501	0
85	1	J	63494	0
85	2	h	63494	0
86	1	J	63506	0
86	2	i	63506	0
87	1	J	63499	0
87	2	j	63499	0
88	1	J	63474	0
88	2	k	63474	0
89	1	J	63508	0
89	2	l	63508	0
90	1	K	63486	0
90	2	L	63486	0
91	1	K	63480	0
91	2	g	63480	0
92	1	K	63487	0
92	2	h	63487	0
93	1	K	63488	0
93	2	i	63488	0
94	1	K	63486	0
94	2	j	63486	0
95	1	K	63484	0
95	2	k	63484	0
96	1	K	63477	0
96	2	l	63477	0
97	1	L	63490	0
97	2	g	63490	0
98	1	L	63480	0
98	2	h	63480	0
99	1	L	63489	0
99	2	i	63489	0
100	1	L	63492	0
100	2	j	63492	0
101	1	L	63487	0
101	2	k	63487	0
102	1	L	63479	0
102	2	l	63479	0
103	1	M	3355	0
103	2	N	3355	0
104	1	M	3351	0
104	2	O	3351	0
105	1	M	3352	0
105	2	P	3352	0
106	1	M	3354	0
106	2	Q	3354	0
107	1	M	3355	0
107	2	R	3355	0
108	1	M	3354	0
108	2	m	3354	0
109	1	M	3354	0
109	2	n	3354	0
110	1	M	3354	0
110	2	o	3354	0
111	1	M	3354	0
111	2	p	3354	0
112	1	M	3354	0
112	2	q	3354	0
113	1	M	3353	0
113	2	r	3353	0
114	1	N	3352	0
114	2	O	3352	0
115	1	N	3353	0
115	2	P	3353	0
116	1	N	3355	0
116	2	Q	3355	0
117	1	N	3356	0
117	2	R	3356	0
118	1	N	3355	0
118	2	m	3355	0
119	1	N	3355	0
119	2	n	3355	0
120	1	N	3356	0
120	2	o	3356	0
121	1	N	3355	0
121	2	p	3355	0
122	1	N	3355	0
122	2	q	3355	0
123	1	N	3353	0
123	2	r	3353	0
124	1	O	3351	0
124	2	P	3351	0
125	1	O	3352	0
125	2	Q	3352	0
126	1	O	3352	0
126	2	R	3352	0
127	1	O	3353	0
127	2	m	3353	0
128	1	O	3353	0
128	2	n	3353	0
129	1	O	3352	0
129	2	o	3352	0
130	1	O	3353	0
130	2	p	3353	0
131	1	O	3351	0
131	2	q	3351	0
132	1	O	3351	0
132	2	r	3351	0
133	1	P	3353	0
133	2	Q	3353	0
134	1	P	3354	0
134	2	R	3354	0
135	1	P	3352	0
135	2	m	3352	0
136	1	P	3353	0
136	2	n	3353	0
137	1	P	3353	0
137	2	o	3353	0
138	1	P	3352	0
138	2	p	3352	0
139	1	P	3352	0
139	2	q	3352	0
140	1	P	3352	0
140	2	r	3352	0
141	1	Q	3355	0
141	2	R	3355	0
142	1	Q	3354	0
142	2	m	3354	0
143	1	Q	3355	0
143	2	n	3355	0
144	1	Q	3354	0
144	2	o	3354	0
145	1	Q	3354	0
145	2	p	3354	0
146	1	Q	3354	0
146	2	q	3354	0
147	1	Q	3353	0
147	2	r	3353	0
148	1	R	3355	0
148	2	m	3355	0
149	1	R	3355	0
149	2	n	3355	0
150	1	R	3356	0
150	2	o	3356	0
151	1	R	3355	0
151	2	p	3355	0
152	1	R	3355	0
152	2	q	3355	0
153	1	R	3353	0
153	2	r	3353	0
154	1	a	54874	0.02
154	2	b	54874	0.02
155	1	a	54861	0.02
155	2	c	54861	0.02
156	1	a	54869	0.02
156	2	d	54869	0.02
157	1	a	54890	0.02
157	2	e	54890	0.02
158	1	a	54853	0.02
158	2	f	54853	0.02
159	1	b	54916	0.02
159	2	c	54916	0.02
160	1	b	54893	0.02
160	2	d	54893	0.02
161	1	b	54915	0.02
161	2	e	54915	0.02
162	1	b	54907	0.02
162	2	f	54907	0.02
163	1	c	54952	0.02
163	2	d	54952	0.02
164	1	c	54960	0.02
164	2	e	54960	0.02
165	1	c	54925	0.02
165	2	f	54925	0.02
166	1	d	54964	0.02
166	2	e	54964	0.02
167	1	d	54932	0.02
167	2	f	54932	0.02
168	1	e	54937	0.02
168	2	f	54937	0.02
169	1	g	63478	0
169	2	h	63478	0
170	1	g	63496	0
170	2	i	63496	0
171	1	g	63489	0
171	2	j	63489	0
172	1	g	63477	0
172	2	k	63477	0
173	1	g	63486	0
173	2	l	63486	0
174	1	h	63495	0
174	2	i	63495	0
175	1	h	63491	0
175	2	j	63491	0
176	1	h	63476	0
176	2	k	63476	0
177	1	h	63474	0
177	2	l	63474	0
178	1	i	63491	0
178	2	j	63491	0
179	1	i	63481	0
179	2	k	63481	0
180	1	i	63479	0
180	2	l	63479	0
181	1	j	63483	0
181	2	k	63483	0
182	1	j	63481	0
182	2	l	63481	0
183	1	k	63467	0
183	2	l	63467	0
184	1	m	3355	0
184	2	n	3355	0
185	1	m	3356	0
185	2	o	3356	0
186	1	m	3356	0
186	2	p	3356	0
187	1	m	3354	0
187	2	q	3354	0
188	1	m	3352	0
188	2	r	3352	0
189	1	n	3355	0
189	2	o	3355	0
190	1	n	3355	0
190	2	p	3355	0
191	1	n	3354	0
191	2	q	3354	0
192	1	n	3353	0
192	2	r	3353	0
193	1	o	3355	0
193	2	p	3355	0
194	1	o	3354	0
194	2	q	3354	0
195	1	o	3352	0
195	2	r	3352	0
196	1	p	3354	0
196	2	q	3354	0
197	1	p	3352	0
197	2	r	3352	0
198	1	q	3352	0
198	2	r	3352	0

LS refinement shell

Resolution: 4.6→4.719 Å / R_factor R_free error: 0 / Total num. of bins used: 20

	Rfactor	Num. reflection	% reflection
Rfree	0.375	1667	-
Rwork	0.374	32216	-
all	-	33883	-
obs	-	-	99.73 %