#256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1)
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Assembly
Deposited unit
H: FAB HEAVY CHAIN I: FAB HEAVY CHAIN J: FAB HEAVY CHAIN K: FAB HEAVY CHAIN L: FAB LIGHT CHAIN M: FAB LIGHT CHAIN O: FAB LIGHT CHAIN T: FAB LIGHT CHAIN hetero molecules
Mass: 23906.707 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lama glama (llama) / Production host: Cricetulus griseus (Chinese hamster)
#2: Antibody
FABLIGHTCHAIN
Mass: 23524.033 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lama glama (llama) / Production host: Cricetulus griseus (Chinese hamster)
Mass: 18.015 Da / Num. of mol.: 316 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.21 Å3/Da / Density % sol: 44.33 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 50 mM Na-Ac pH 5.5, 150 mM NaCl with 1.5 M (NH4)2SO4, 150 mM sodium citrate
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Resolution: 1.9→47.6 Å / Cor.coef. Fo:Fc: 0.976 / Cor.coef. Fo:Fc free: 0.959 / SU B: 11.821 / SU ML: 0.141 / Cross valid method: THROUGHOUT / ESU R: 0.622 / ESU R Free: 0.145 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22802
6624
5 %
RANDOM
Rwork
0.16872
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obs
0.17173
125855
99.7 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å