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Yorodumi- PDB-6qbw: Structure of the HTLV-2 integrase catalytic core domain in comple... -
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-Basic information
Entry | Database: PDB / ID: 6qbw | ||||||
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Title | Structure of the HTLV-2 integrase catalytic core domain in complex with calcium | ||||||
Components | integrase | ||||||
Keywords | TRANSFERASE / retrovirus / deltaretrovirus / integration / strand-transfer / nucleotidyltransferase | ||||||
Function / homology | Function and homology information Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / DNA integration / RNA-directed DNA polymerase / viral genome integration into host DNA / establishment of integrated proviral latency / RNA-directed DNA polymerase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / RNA-DNA hybrid ribonuclease activity / viral nucleocapsid ...Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / ribonuclease H / DNA integration / RNA-directed DNA polymerase / viral genome integration into host DNA / establishment of integrated proviral latency / RNA-directed DNA polymerase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / RNA-DNA hybrid ribonuclease activity / viral nucleocapsid / DNA recombination / Hydrolases; Acting on ester bonds / nucleic acid binding / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / symbiont-mediated suppression of host gene expression / structural molecule activity / proteolysis / DNA binding / zinc ion binding Similarity search - Function | ||||||
Biological species | Human T-cell leukemia virus 2 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Barski, M.S. / Maertens, G.N. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Nat Commun / Year: 2020 Title: Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56γ. Authors: Michał S Barski / Jordan J Minnell / Zuzana Hodakova / Valerie E Pye / Andrea Nans / Peter Cherepanov / Goedele N Maertens / Abstract: Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor ...Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor disease, unless a therapy becomes available. A key step in the establishment of infection is the integration of viral genetic material into the host genome, catalysed by the retroviral integrase (IN) enzyme. Here, we use X-ray crystallography and single-particle cryo-electron microscopy to determine the structure of the functional deltaretroviral IN assembled on viral DNA ends and bound to the B56γ subunit of its human host factor, protein phosphatase 2 A. The structure reveals a tetrameric IN assembly bound to two molecules of the phosphatase via a conserved short linear motif. Insight into the deltaretroviral intasome and its interaction with the host will be crucial for understanding the pattern of integration events in infected individuals and therefore bears important clinical implications. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6qbw.cif.gz | 44.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6qbw.ent.gz | 29.7 KB | Display | PDB format |
PDBx/mmJSON format | 6qbw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qb/6qbw ftp://data.pdbj.org/pub/pdb/validation_reports/qb/6qbw | HTTPS FTP |
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-Related structure data
Related structure data | 6qbtSC 6qbvC 6tjuC 6toqC 7pelC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19178.967 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human T-cell leukemia virus 2 / Gene: pol / Production host: Escherichia coli (E. coli) / Variant (production host): Rosetta2 / References: UniProt: Q82441, UniProt: P03363*PLUS |
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#2: Chemical | ChemComp-CA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.06 Å3/Da / Density % sol: 65.93 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 100 mM Tris pH 8, 18% PEG 6000, 250 calcium chloride dihydrate |
-Data collection
Diffraction | Mean temperature: 291 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: May 11, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50.12 Å / Num. obs: 9932 / % possible obs: 100 % / Redundancy: 1.8 % / Rmerge(I) obs: 0.028 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 1872 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6QBT Resolution: 2.4→50.12 Å / Cross valid method: FREE R-VALUE
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Solvent computation | Ion probe radii: 0.9 Å / VDW probe radii: 1.2 Å | ||||||||||||||||
Displacement parameters | Biso mean: 53.312 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→50.12 Å
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