+
Open data
-
Basic information
Entry | Database: PDB / ID: 6q25 | ||||||
---|---|---|---|---|---|---|---|
Title | Coiled-coil Trimer with Glu:Leu:Lys Triad | ||||||
![]() | Coiled-coil Trimer with Glu:Leu:Lys Triad | ||||||
![]() | DE NOVO PROTEIN / Trimer / Helix | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() | ||||||
![]() | Smith, M.S. / Stern, K.L. / Billings, W.M. / Price, J.L. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Context-Dependent Stabilizing Interactions among Solvent-Exposed Residues along the Surface of a Trimeric Helix Bundle. Authors: Stern, K.L. / Smith, M.S. / Billings, W.M. / Loftus, T.J. / Conover, B.M. / Della Corte, D. / Price, J.L. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 35.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 27.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 408.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 408.4 KB | Display | |
Data in XML | ![]() | 5.6 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6os8C ![]() 6osdC ![]() 6ov9C ![]() 6ovsC ![]() 6ovuC ![]() 6ovvC ![]() 6q1wC ![]() 6q22C ![]() 6u47C ![]() 6v4yC ![]() 6v50C ![]() 6v57C ![]() 6v58C ![]() 6v5gC ![]() 6v5iC ![]() 6v5jC C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein/peptide | Mass: 3866.458 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Water | ChemComp-HOH / | Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 36.19 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 50% PEG200, 100 mM HEPES free acid/NaOH, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: APEX II CCD / Detector: CCD / Date: Sep 29, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5406 Å / Relative weight: 1 |
Reflection | Resolution: 1.893→17.409 Å / Num. obs: 4584 / % possible obs: 98.94 % / Redundancy: 4.9 % / CC1/2: 0.948 / Rmerge(I) obs: 0.191 / Rpim(I) all: 0.087 / Rrim(I) all: 0.211 / Net I/σ(I): 9.9 |
Reflection shell | Resolution: 1.893→1.961 Å / Rmerge(I) obs: 0.532 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 469 / CC1/2: 0.668 / Rpim(I) all: 0.38 / Rrim(I) all: 0.658 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 52.46 Å2 / Biso mean: 16.1865 Å2 / Biso min: 3.83 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.893→17.409 Å
| ||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
|