温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 詳細: The protein (about 5 mg/mL) was preincubated with the inhibitor (dissolved in dimethylsulfoxide) at a molar ratio of 1:5. Crystals were grown from 0.1 m sodium acetate buffer, pH 4.6 and 2M ...詳細: The protein (about 5 mg/mL) was preincubated with the inhibitor (dissolved in dimethylsulfoxide) at a molar ratio of 1:5. Crystals were grown from 0.1 m sodium acetate buffer, pH 4.6 and 2M NaCl as precipitant. Crystals were cryo-cooled in liquid nitrogen after soaking in 30% glycerol to prevent freezing.
解像度: 1.5→33.52 Å / Cor.coef. Fo:Fc: 0.973 / Cor.coef. Fo:Fc free: 0.964 / SU B: 2.348 / SU ML: 0.038 / 交差検証法: THROUGHOUT / ESU R: 0.064 / ESU R Free: 0.06 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS