National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
5SC1AI114843
米国
National Institutes of Health/National Institute on Minority Health and Health Disparities (NIH/NIMHD)
5G12MD007603
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM103310
米国
引用
ジャーナル: Virology / 年: 2019 タイトル: Structural characterization of the PCV2d virus-like particle at 3.3 Å resolution reveals differences to PCV2a and PCV2b capsids, a tetranucleotide, and an N-terminus near the icosahedral 3-fold axes. 著者: Reza Khayat / Ke Wen / Aleksandra Alimova / Boris Gavrilov / Al Katz / Jose M Galarza / Paul Gottlieb / 要旨: Porcine circovirus 2 (PCV2) has a major impact on the swine industry. Eight PCV2 genotypes (a-h) have been identified using capsid sequence analysis. PCV2d has been designated as the emerging ...Porcine circovirus 2 (PCV2) has a major impact on the swine industry. Eight PCV2 genotypes (a-h) have been identified using capsid sequence analysis. PCV2d has been designated as the emerging genotype. The cryo-electron microscopy molecular envelope of PCV2d virus-like particles identifies differences between PCV2a, b and d genotypes that accompany the emergence of PCV2b from PCV2a, and PCV2d from PCV2b. These differences indicate that sequence analysis of genotypes is insufficient, and that it is important to determine the PCV2 capsid structure as the virus evolves. Structure-based sequence comparison demonstrate that each genotype possesses a unique combination of amino acids located on the surface of the capsid that undergo substitution. We also demonstrate that the capsid N-terminus moves in response to increasing amount of nucleic acid packaged into the capsid. Furthermore, we model a tetranucleotide between the 5- and 2-fold axes of symmetry that appears to be responsible for capsid stability.
A0: Capsid protein A1: Capsid protein A2: Capsid protein A3: Capsid protein A4: Capsid protein A5: Capsid protein A6: Capsid protein A7: Capsid protein A8: Capsid protein A9: Capsid protein AA: Capsid protein AB: Capsid protein AC: Capsid protein AD: Capsid protein AE: Capsid protein AF: Capsid protein AG: Capsid protein AH: Capsid protein AI: Capsid protein AJ: Capsid protein AK: Capsid protein AL: Capsid protein AM: Capsid protein AN: Capsid protein AO: Capsid protein AP: Capsid protein AQ: Capsid protein AR: Capsid protein AS: Capsid protein AT: Capsid protein AU: Capsid protein AV: Capsid protein AW: Capsid protein AX: Capsid protein AY: Capsid protein AZ: Capsid protein Aa: Capsid protein Ab: Capsid protein Ac: Capsid protein Ad: Capsid protein Ae: Capsid protein Af: Capsid protein Ag: Capsid protein Ah: Capsid protein Ai: Capsid protein Aj: Capsid protein Ak: Capsid protein Al: Capsid protein Am: Capsid protein An: Capsid protein Ao: Capsid protein Ap: Capsid protein Aq: Capsid protein Ar: Capsid protein As: Capsid protein At: Capsid protein Au: Capsid protein Av: Capsid protein Aw: Capsid protein Ax: Capsid protein B0: DNA (5'-D(P*CP*CP*GP*G)-3') B1: DNA (5'-D(P*CP*CP*GP*G)-3') B2: DNA (5'-D(P*CP*CP*GP*G)-3') B3: DNA (5'-D(P*CP*CP*GP*G)-3') B4: DNA (5'-D(P*CP*CP*GP*G)-3') B5: DNA (5'-D(P*CP*CP*GP*G)-3') B6: DNA (5'-D(P*CP*CP*GP*G)-3') B7: DNA (5'-D(P*CP*CP*GP*G)-3') B8: DNA (5'-D(P*CP*CP*GP*G)-3') B9: DNA (5'-D(P*CP*CP*GP*G)-3') BA: DNA (5'-D(P*CP*CP*GP*G)-3') BB: DNA (5'-D(P*CP*CP*GP*G)-3') BC: DNA (5'-D(P*CP*CP*GP*G)-3') BD: DNA (5'-D(P*CP*CP*GP*G)-3') BE: DNA (5'-D(P*CP*CP*GP*G)-3') BF: DNA (5'-D(P*CP*CP*GP*G)-3') BG: DNA (5'-D(P*CP*CP*GP*G)-3') BH: DNA (5'-D(P*CP*CP*GP*G)-3') BI: DNA (5'-D(P*CP*CP*GP*G)-3') BJ: DNA (5'-D(P*CP*CP*GP*G)-3') BK: DNA (5'-D(P*CP*CP*GP*G)-3') BL: DNA (5'-D(P*CP*CP*GP*G)-3') BM: DNA (5'-D(P*CP*CP*GP*G)-3') BN: DNA (5'-D(P*CP*CP*GP*G)-3') BO: DNA (5'-D(P*CP*CP*GP*G)-3') BP: DNA (5'-D(P*CP*CP*GP*G)-3') BQ: DNA (5'-D(P*CP*CP*GP*G)-3') BR: DNA (5'-D(P*CP*CP*GP*G)-3') BS: DNA (5'-D(P*CP*CP*GP*G)-3') BT: DNA (5'-D(P*CP*CP*GP*G)-3') BU: DNA (5'-D(P*CP*CP*GP*G)-3') BV: DNA (5'-D(P*CP*CP*GP*G)-3') BW: DNA (5'-D(P*CP*CP*GP*G)-3') BX: DNA (5'-D(P*CP*CP*GP*G)-3') BY: DNA (5'-D(P*CP*CP*GP*G)-3') BZ: DNA (5'-D(P*CP*CP*GP*G)-3') Ba: DNA (5'-D(P*CP*CP*GP*G)-3') Bb: DNA (5'-D(P*CP*CP*GP*G)-3') Bc: DNA (5'-D(P*CP*CP*GP*G)-3') Bd: DNA (5'-D(P*CP*CP*GP*G)-3') Be: DNA (5'-D(P*CP*CP*GP*G)-3') Bf: DNA (5'-D(P*CP*CP*GP*G)-3') Bg: DNA (5'-D(P*CP*CP*GP*G)-3') Bh: DNA (5'-D(P*CP*CP*GP*G)-3') Bi: DNA (5'-D(P*CP*CP*GP*G)-3') Bj: DNA (5'-D(P*CP*CP*GP*G)-3') Bk: DNA (5'-D(P*CP*CP*GP*G)-3') Bl: DNA (5'-D(P*CP*CP*GP*G)-3') Bm: DNA (5'-D(P*CP*CP*GP*G)-3') Bn: DNA (5'-D(P*CP*CP*GP*G)-3') Bo: DNA (5'-D(P*CP*CP*GP*G)-3') Bp: DNA (5'-D(P*CP*CP*GP*G)-3') Bq: DNA (5'-D(P*CP*CP*GP*G)-3') Br: DNA (5'-D(P*CP*CP*GP*G)-3') Bs: DNA (5'-D(P*CP*CP*GP*G)-3') Bt: DNA (5'-D(P*CP*CP*GP*G)-3') Bu: DNA (5'-D(P*CP*CP*GP*G)-3') Bv: DNA (5'-D(P*CP*CP*GP*G)-3') Bw: DNA (5'-D(P*CP*CP*GP*G)-3') Bx: DNA (5'-D(P*CP*CP*GP*G)-3')