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- PDB-6nlq: Human Mitochondrial Alanyl-tRNA Synthetase C-terminal domain -

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Basic information

Entry
Database: PDB / ID: 6nlq
TitleHuman Mitochondrial Alanyl-tRNA Synthetase C-terminal domain
ComponentsAlanine--tRNA ligase, mitochondrial
KeywordsLIGASE / Alanyl-tRNA synthetase / C-terminal domain / alpha-beta domain
Function / homology
Function and homology information


mitochondrial alanyl-tRNA aminoacylation / alanine-tRNA ligase / alanine-tRNA ligase activity / alanyl-tRNA aminoacylation / Mitochondrial tRNA aminoacylation / aminoacyl-tRNA editing activity / tRNA binding / mitochondrion / zinc ion binding / ATP binding
Similarity search - Function
Alanine-tRNA ligase, eukaryota/bacteria / Alanine-tRNA ligase, class IIc / Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily / Alanyl-tRNA synthetase, class IIc, N-terminal / Alanyl-tRNA synthetase, class IIc, core domain / tRNA synthetases class II (A) / Alanyl-transfer RNA synthetases family profile. / Threonyl/alanyl tRNA synthetase, SAD / Threonyl and Alanyl tRNA synthetase second additional domain / Threonyl and Alanyl tRNA synthetase second additional domain ...Alanine-tRNA ligase, eukaryota/bacteria / Alanine-tRNA ligase, class IIc / Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily / Alanyl-tRNA synthetase, class IIc, N-terminal / Alanyl-tRNA synthetase, class IIc, core domain / tRNA synthetases class II (A) / Alanyl-transfer RNA synthetases family profile. / Threonyl/alanyl tRNA synthetase, SAD / Threonyl and Alanyl tRNA synthetase second additional domain / Threonyl and Alanyl tRNA synthetase second additional domain / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) / Translation protein, beta-barrel domain superfamily
Similarity search - Domain/homology
Alanine--tRNA ligase, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.15 Å
AuthorsKuhle, B. / Schimmel, P.
CitationJournal: Nat Commun / Year: 2020
Title: Relaxed sequence constraints favor mutational freedom in idiosyncratic metazoan mitochondrial tRNAs.
Authors: Kuhle, B. / Chihade, J. / Schimmel, P.
History
DepositionJan 8, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 15, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Database references / Derived calculations / Structure summary
Category: citation / citation_author ...citation / citation_author / pdbx_struct_conn_angle / struct / struct_conn
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_alt_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_symmetry / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_alt_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct.title / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_ptnr1_label_alt_id / _struct_conn.pdbx_ptnr2_label_alt_id / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry
Revision 1.2Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Alanine--tRNA ligase, mitochondrial
B: Alanine--tRNA ligase, mitochondrial
C: Alanine--tRNA ligase, mitochondrial
D: Alanine--tRNA ligase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,2277
Polymers47,1064
Non-polymers1203
Water8,863492
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A: Alanine--tRNA ligase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,8172
Polymers11,7771
Non-polymers401
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Alanine--tRNA ligase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,8172
Polymers11,7771
Non-polymers401
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Alanine--tRNA ligase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,8172
Polymers11,7771
Non-polymers401
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Alanine--tRNA ligase, mitochondrial


Theoretical massNumber of molelcules
Total (without water)11,7771
Polymers11,7771
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)55.190, 66.950, 56.480
Angle α, β, γ (deg.)90.000, 116.420, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resseq 885:886 or resseq 890 or resseq...
21(chain B and (resseq 885:886 or resseq 890 or resseq...
31(chain C and (resseq 885:886 or resseq 890 or resseq...
41(chain D and (resseq 885:886 or resseq 890 or resseq...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and (resseq 885:886 or resseq 890 or resseq...A885 - 886
121(chain A and (resseq 885:886 or resseq 890 or resseq...A890
131(chain A and (resseq 885:886 or resseq 890 or resseq...A3
141(chain A and (resseq 885:886 or resseq 890 or resseq...A881 - 985
151(chain A and (resseq 885:886 or resseq 890 or resseq...A881 - 985
161(chain A and (resseq 885:886 or resseq 890 or resseq...A881 - 985
171(chain A and (resseq 885:886 or resseq 890 or resseq...A881 - 985
181(chain A and (resseq 885:886 or resseq 890 or resseq...A881 - 985
191(chain A and (resseq 885:886 or resseq 890 or resseq...A881 - 985
211(chain B and (resseq 885:886 or resseq 890 or resseq...B885 - 886
221(chain B and (resseq 885:886 or resseq 890 or resseq...B890
231(chain B and (resseq 885:886 or resseq 890 or resseq...B892 - 893
241(chain B and (resseq 885:886 or resseq 890 or resseq...B895
251(chain B and (resseq 885:886 or resseq 890 or resseq...B880 - 985
261(chain B and (resseq 885:886 or resseq 890 or resseq...B880 - 985
271(chain B and (resseq 885:886 or resseq 890 or resseq...B880 - 985
281(chain B and (resseq 885:886 or resseq 890 or resseq...B880 - 985
291(chain B and (resseq 885:886 or resseq 890 or resseq...B880 - 985
311(chain C and (resseq 885:886 or resseq 890 or resseq...C885 - 886
321(chain C and (resseq 885:886 or resseq 890 or resseq...C890
331(chain C and (resseq 885:886 or resseq 890 or resseq...C3
341(chain C and (resseq 885:886 or resseq 890 or resseq...C881 - 985
351(chain C and (resseq 885:886 or resseq 890 or resseq...C881 - 985
361(chain C and (resseq 885:886 or resseq 890 or resseq...C881 - 985
371(chain C and (resseq 885:886 or resseq 890 or resseq...C881 - 985
381(chain C and (resseq 885:886 or resseq 890 or resseq...C881 - 985
391(chain C and (resseq 885:886 or resseq 890 or resseq...C881 - 985
411(chain D and (resseq 885:886 or resseq 890 or resseq...D885 - 886
421(chain D and (resseq 885:886 or resseq 890 or resseq...D890
431(chain D and (resseq 885:886 or resseq 890 or resseq...D892 - 893
441(chain D and (resseq 885:886 or resseq 890 or resseq...D881 - 985
451(chain D and (resseq 885:886 or resseq 890 or resseq...D881 - 985
461(chain D and (resseq 885:886 or resseq 890 or resseq...D881 - 985
471(chain D and (resseq 885:886 or resseq 890 or resseq...D881 - 985
481(chain D and (resseq 885:886 or resseq 890 or resseq...D881 - 985
491(chain D and (resseq 885:886 or resseq 890 or resseq...D881 - 985

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Components

#1: Protein
Alanine--tRNA ligase, mitochondrial / Alanyl-tRNA synthetase / AlaRS


Mass: 11776.592 Da / Num. of mol.: 4 / Fragment: C-terminal domain (UNP residues 873-985)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AARS2, AARSL, KIAA1270 / Plasmid: pET21 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q5JTZ9, alanine-tRNA ligase
#2: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Ca
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 492 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.98 Å3/Da / Density % sol: 38 %
Crystal growTemperature: 298 K / Method: evaporation / pH: 7
Details: 0.1 M Tris-HCl, 100 mM calcium chloride, 18% PEG3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.97946 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 18, 2017
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97946 Å / Relative weight: 1
ReflectionResolution: 1.15→38.709 Å / Num. obs: 123979 / % possible obs: 95 % / Redundancy: 3.251 % / Biso Wilson estimate: 11.34 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.036 / Rrim(I) all: 0.043 / Χ2: 1.033 / Net I/σ(I): 20.32 / Num. measured all: 403060 / Scaling rejects: 265
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
1.15-1.182.6830.156.7918355960068410.9640.18971.3
1.18-1.213.0940.1338.328273931191370.9750.16298.1
1.21-1.253.1420.1149.5328099916889420.9820.13997.5
1.25-1.293.1580.09611.0727178887886070.9870.11696.9
1.29-1.333.2070.08512.7426171856281610.9890.10395.3
1.33-1.373.3840.07314.8127804832282170.9930.08798.7
1.37-1.433.3730.06316.6426568801178760.9940.07598.3
1.43-1.483.3150.05418.8325016774175460.9950.06497.5
1.48-1.553.2710.04621.4723091738470600.9960.05595.6
1.55-1.633.4090.0424.2723965711570290.9970.04898.8
1.63-1.713.4020.03726.3722557673466310.9970.04498.5
1.71-1.823.3410.03528.0420609638361680.9970.04196.6
1.82-1.943.2140.03129.5218103598856330.9980.03894.1
1.94-2.13.4320.02932.5118768559354680.9980.03497.8
2.1-2.33.3990.02833.5116953515849880.9980.03396.7
2.3-2.573.270.02833.3614749467045100.9980.03396.6
2.57-2.973.2860.02834.1712692411738620.9980.03493.8
2.97-3.643.4340.03335.4711587350733740.9960.03996.2
3.64-5.143.1160.0433.877901272125360.990.04893.2
5.14-38.7093.3170.04635.054621152813930.9890.05591.2

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Phasing

PhasingMethod: molecular replacement
Phasing MRModel details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation6.17 Å38.71 Å
Translation6.17 Å38.71 Å

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Processing

Software
NameVersionClassificationNB
PHENIXrefinement
XDSdata reduction
XSCALEdata scaling
PHASER2.5.7phasing
PDB_EXTRACT3.24data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 5T5S

5t5s


Resolution: 1.15→38.709 Å / SU ML: 0.06 / Cross valid method: THROUGHOUT / σ(F): 1.38 / Phase error: 15.75
RfactorNum. reflection% reflection
Rfree0.1723 6199 5 %
Rwork0.1585 --
obs0.1592 123978 95.17 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 55.54 Å2 / Biso mean: 18.1394 Å2 / Biso min: 6.99 Å2
Refinement stepCycle: final / Resolution: 1.15→38.709 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3013 0 3 505 3521
Biso mean--10.92 27.01 -
Num. residues----421
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0123543
X-RAY DIFFRACTIONf_angle_d1.4614900
X-RAY DIFFRACTIONf_chiral_restr0.112599
X-RAY DIFFRACTIONf_plane_restr0.01649
X-RAY DIFFRACTIONf_dihedral_angle_d23.0381283
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A948X-RAY DIFFRACTION8.406TORSIONAL
12B948X-RAY DIFFRACTION8.406TORSIONAL
13C948X-RAY DIFFRACTION8.406TORSIONAL
14D948X-RAY DIFFRACTION8.406TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.1502-1.16330.21231160.19732217233354
1.1633-1.17690.18071830.17993472365584
1.1769-1.19130.21452070.17663933414097
1.1913-1.20640.17882140.16954067428198
1.2064-1.22230.19382110.16714014422598
1.2223-1.2390.19062130.16744036424998
1.239-1.25670.15732110.16184008421997
1.2567-1.27550.15282090.16353974418397
1.2755-1.29540.18672070.16243944415196
1.2954-1.31660.17312050.16173881408694
1.3166-1.33930.20282140.16064064427899
1.3393-1.36370.17172120.15774031424399
1.3637-1.38990.16112130.15834058427199
1.3899-1.41830.1712150.15694080429599
1.4183-1.44910.17022130.15314042425598
1.4491-1.48280.1612110.15464008421998
1.4828-1.51990.17822010.14833829403093
1.5199-1.5610.15582150.14634075429099
1.561-1.6070.15842150.14334081429699
1.607-1.65880.15882150.14244085430099
1.6588-1.71810.15722140.14894065427999
1.7181-1.78690.19582120.15464033424597
1.7869-1.86820.16592030.15713860406394
1.8682-1.96670.14942080.15383960416896
1.9667-2.08990.14632130.15094048426198
2.0899-2.25130.1522110.14684010422197
2.2513-2.47780.19072140.16164061427597
2.4778-2.83630.18792040.16793871407594
2.8363-3.5730.18492120.16094021423397
3.573-38.73110.1732080.1663951415993
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.98370.9827-0.60472.2102-0.11951.5134-0.08820.00920.0178-0.2111-0.00140.11730.0658-0.05270.09580.09250.0016-0.00840.0738-0.00690.098416.4893.3897-3.0904
24.1793.46623.18285.79775.23086.5193-0.12380.05230.1683-0.3308-0.07120.2734-0.3853-0.25290.20340.09440.0178-0.00390.0822-0.00310.090711.552811.2603-7.2631
33.17180.14950.10231.85560.24872.35150.0892-0.19830.04830.144-0.050.1260.0708-0.2253-0.03540.0763-0.01410.01670.0790.00040.07348.81515.30312.0868
43.256-0.74710.58684.1033-1.02592.21620.0609-0.2858-0.10940.0944-0.01820.11580.0492-0.1497-0.03960.1159-0.01920.02990.1520.00030.081210.31873.49336.4866
53.7434-0.28470.4013.89591.70273.73030.03190.1543-0.1740.0409-0.160.38480.1476-0.35250.12920.086-0.02330.00350.10820.01680.079-12.63215.787731.2095
61.8167-0.4042-0.05661.83330.31982.49820.0197-0.0094-0.01040.1323-0.0385-0.0860.0333-0.02070.01730.0772-0.0034-0.00150.0760.00040.0672-6.70824.914339.0027
72.6021-0.2089-0.11553.41310.24252.9357-0.00990.0432-0.00060.08280.01310.0198-0.0171-0.15470.01980.0732-0.00320.00350.0855-0.00450.0657-9.40614.944331.3395
82.5021-1.64770.26585.1859-0.0511.3736-0.01480.13050.3204-0.0060.06820.1554-0.4188-0.16370.05660.15410.03670.01080.06890.02740.1193-7.440515.379829.232
95.7387-1.0216-1.95925.42990.49280.6782-0.1760.0766-0.50580.0135-0.0662-0.37320.8210.25490.18610.23710.02790.03010.0875-0.02530.14160.3007-5.136526.6817
102.4424-0.0740.22893.9098-0.22553.0634-0.03150.22030.22-0.1405-0.0409-0.0271-0.1801-0.0880.07420.07260.00420.0050.06630.01740.0664-1.787710.673824.9336
116.535-2.9625-0.97057.13651.99984.20930.09990.3376-0.3062-0.1305-0.14990.30960.1568-0.31270.030.1416-0.039-0.03830.1642-0.00990.0859-9.7743.733819.8295
126.4345-0.70040.0856.36980.21853.46550.1331-0.07590.0557-0.06360.0362-0.6896-0.17420.3437-0.19940.0899-0.01890.01630.1110.04140.070316.60190.865345.4318
131.77630.15880.13431.9989-0.42991.70510.04560.01920.04770.0558-0.0248-0.0149-0.00790.0202-0.01930.05720.00070.00180.05970.01120.069211.70262.281443.5433
143.0272-0.0296-0.5893.36010.02916.10260.02480.2204-0.2137-0.21290.0120.10690.303-0.4267-0.06910.1172-0.0132-0.01840.08410.00370.11415.5924-8.713143.7353
154.4882-0.78460.80423.3788-0.47616.46270.0502-0.0790.11360.24580.0219-0.1087-0.1351-0.1544-0.04160.1004-0.00660.01680.06040.02730.10345.7703-1.806652.171
163.83691.45382.20536.93773.48927.15540.2030.0699-0.21960.02290.1585-0.45870.2610.2165-0.34630.140.0326-0.01110.09310.03270.137614.7917-11.039748.4548
172.94080.6659-0.38924.0876-0.544.7947-0.0405-0.01860.0373-0.06410.05430.11320.15420.0395-0.00660.08020.0149-0.01270.0678-0.00850.094616.8657-21.62516.9454
183.43111.6338-1.20243.3055-0.33482.86280.04750.09920.0829-0.2156-0.09330.38980.0212-0.25210.0410.09510.0246-0.02180.1163-0.02930.138710.0424-22.89149.8153
195.58130.14450.56262.42740.38263.3199-0.0705-0.07450.3492-0.00710.0146-0.1558-0.20980.18330.05240.09060.00880.00090.0841-0.01940.104818.6418-14.449317.1245
203.97350.82740.24415.6857-2.94271.63130.0564-0.261-0.22930.2847-0.14871.36640.0981-1.25230.01950.1347-0.02730.0550.3708-0.09810.3735-1.1195-18.703417.8566
214.71822.0811.71842.85662.13954.0276-0.2126-0.37130.30160.09250.08130.0163-0.3549-0.06240.12440.17430.04710.00510.1518-0.03920.11389.8681-10.254221.8183
223.30660.76111.23283.29180.39493.7454-0.2108-0.21720.35380.12150.0902-0.07-0.25510.12150.10990.11860.03190.00560.1036-0.03660.101113.2438-9.274817.7367
238.546-0.7470.09852.59170.31013.2579-0.378-1.1408-0.56150.36990.2680.21810.3336-0.25280.04750.24830.0620.05880.28320.03190.128410.3684-18.215627.3373
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 881 through 892 )A881 - 892
2X-RAY DIFFRACTION2chain 'A' and (resid 893 through 906 )A893 - 906
3X-RAY DIFFRACTION3chain 'A' and (resid 907 through 949 )A907 - 949
4X-RAY DIFFRACTION4chain 'A' and (resid 950 through 985 )A950 - 985
5X-RAY DIFFRACTION5chain 'B' and (resid 880 through 892 )B880 - 892
6X-RAY DIFFRACTION6chain 'B' and (resid 893 through 906 )B893 - 906
7X-RAY DIFFRACTION7chain 'B' and (resid 907 through 918 )B907 - 918
8X-RAY DIFFRACTION8chain 'B' and (resid 919 through 928 )B919 - 928
9X-RAY DIFFRACTION9chain 'B' and (resid 929 through 938 )B929 - 938
10X-RAY DIFFRACTION10chain 'B' and (resid 939 through 969 )B939 - 969
11X-RAY DIFFRACTION11chain 'B' and (resid 970 through 985 )B970 - 985
12X-RAY DIFFRACTION12chain 'C' and (resid 881 through 892 )C881 - 892
13X-RAY DIFFRACTION13chain 'C' and (resid 893 through 928 )C893 - 928
14X-RAY DIFFRACTION14chain 'C' and (resid 929 through 949 )C929 - 949
15X-RAY DIFFRACTION15chain 'C' and (resid 950 through 969 )C950 - 969
16X-RAY DIFFRACTION16chain 'C' and (resid 970 through 985 )C970 - 985
17X-RAY DIFFRACTION17chain 'D' and (resid 881 through 892 )D881 - 892
18X-RAY DIFFRACTION18chain 'D' and (resid 893 through 910 )D893 - 910
19X-RAY DIFFRACTION19chain 'D' and (resid 911 through 928 )D911 - 928
20X-RAY DIFFRACTION20chain 'D' and (resid 929 through 938 )D929 - 938
21X-RAY DIFFRACTION21chain 'D' and (resid 939 through 949 )D939 - 949
22X-RAY DIFFRACTION22chain 'D' and (resid 950 through 969 )D950 - 969
23X-RAY DIFFRACTION23chain 'D' and (resid 970 through 985 )D970 - 985

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