- PDB-6ndx: Lysinoalanine cross-linked FlgE dimer from Treponema denticola -
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Open data
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Basic information
Entry
Database: PDB / ID: 6ndx
Title
Lysinoalanine cross-linked FlgE dimer from Treponema denticola
Components
(Flagellar hook protein FlgE) x 2
Keywords
MOTOR PROTEIN / hook / lysinoalanine / crosslinking / spirochetes / periodontal disease / FlgE / dehydroalanine
Function / homology
Function and homology information
bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility Similarity search - Function
Flagellar hook protein FlgE superfamily / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / : / Flagellar hook protein FlgE/F/G D1 domain / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal ...Flagellar hook protein FlgE superfamily / Flagellar hook protein FlgE / Flagellar basal body protein FlaE D2 domain / Flagellar hook-basal body protein, FlgE/F/G / Flagellar hook-basal body protein, FlgE/F/G-like / : / Flagellar hook protein FlgE/F/G D1 domain / Flagellar basal body rod protein, conserved site / Flagella basal body rod proteins signature. / Flagellar basal body rod protein, N-terminal / Flagellar basal-body/hook protein, C-terminal domain / Flagella basal body rod protein / Flagellar basal body rod FlgEFG protein C-terminal Similarity search - Domain/homology
Mass: 35533.832 Da / Num. of mol.: 2 / Mutation: C178A Source method: isolated from a genetically manipulated source Details: FlgE(N91-S423) degraded prior to crystallization, N and C termini are missing from structure Source: (gene. exp.) Treponema denticola (bacteria) / Gene: flgE / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9RQB6
#2: Protein
FlagellarhookproteinFlgE
Mass: 18957.521 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: 4 residues on C-termini missing from density, D-alanine present instead of cysteine Source: (gene. exp.) Treponema denticola (bacteria) / Gene: flgE / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9RQB6
Mass: 18.015 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Formula: H2O
Has protein modification
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.9 Å3/Da / Density % sol: 57.53 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop Details: crystallized in two conditions: (1) 100 mM Tris pH 8.5, 20% (w/v) PEG8000, 200 mM magnesium chloride and (2) 200 mM magnesium acetate, 20% PEG3350 (w/v)
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Data collection
Diffraction
Mean temperature: 77 K / Serial crystal experiment: N
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