+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6n4p | ||||||
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タイトル | RQEFEV, crystal structure of the N-terminal segment RQEFEV from protein tau | ||||||
要素 | Microtubule-associated protein tauタウタンパク質 | ||||||
キーワード | PROTEIN FIBRIL / Amyloid (アミロイド) / tau / Alzheimer's Disease (アルツハイマー病) / tauopathy (タウオパチー) / MAPT (タウタンパク質) | ||||||
機能・相同性 | 機能・相同性情報 plus-end-directed organelle transport along microtubule / 軸索輸送 / histone-dependent DNA binding / neurofibrillary tangle assembly / positive regulation of diacylglycerol kinase activity / negative regulation of establishment of protein localization to mitochondrion / neurofibrillary tangle / positive regulation of protein localization to synapse / microtubule lateral binding / tubulin complex ...plus-end-directed organelle transport along microtubule / 軸索輸送 / histone-dependent DNA binding / neurofibrillary tangle assembly / positive regulation of diacylglycerol kinase activity / negative regulation of establishment of protein localization to mitochondrion / neurofibrillary tangle / positive regulation of protein localization to synapse / microtubule lateral binding / tubulin complex / phosphatidylinositol bisphosphate binding / main axon / regulation of long-term synaptic depression / negative regulation of kinase activity / negative regulation of tubulin deacetylation / generation of neurons / regulation of chromosome organization / positive regulation of protein localization / rRNA metabolic process / internal protein amino acid acetylation / regulation of mitochondrial fission / intracellular distribution of mitochondria / axonal transport of mitochondrion / axon development / central nervous system neuron development / regulation of microtubule polymerization / 微小管 / minor groove of adenine-thymine-rich DNA binding / lipoprotein particle binding / dynactin binding / glial cell projection / negative regulation of mitochondrial membrane potential / apolipoprotein binding / protein polymerization / negative regulation of mitochondrial fission / axolemma / Caspase-mediated cleavage of cytoskeletal proteins / regulation of microtubule polymerization or depolymerization / positive regulation of axon extension / Activation of AMPK downstream of NMDARs / regulation of microtubule cytoskeleton organization / supramolecular fiber organization / stress granule assembly / regulation of cellular response to heat / cytoplasmic microtubule organization / regulation of calcium-mediated signaling / positive regulation of microtubule polymerization / axon cytoplasm / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / synapse assembly / phosphatidylinositol binding / nuclear periphery / cellular response to nerve growth factor stimulus / positive regulation of superoxide anion generation / protein phosphatase 2A binding / regulation of autophagy / astrocyte activation / response to lead ion / synapse organization / microglial cell activation / Hsp90 protein binding / regulation of synaptic plasticity / PKR-mediated signaling / protein homooligomerization / 記憶 / cellular response to reactive oxygen species / microtubule cytoskeleton organization / SH3 domain binding / cytoplasmic ribonucleoprotein granule / activation of cysteine-type endopeptidase activity involved in apoptotic process / neuron projection development / microtubule cytoskeleton / cell-cell signaling / protein-macromolecule adaptor activity / actin binding / single-stranded DNA binding / cellular response to heat / cell body / protein-folding chaperone binding / 成長円錐 / microtubule binding / double-stranded DNA binding / 微小管 / sequence-specific DNA binding / amyloid fibril formation / 樹状突起スパイン / learning or memory / nuclear speck / neuron projection / 脂質ラフト / 神経繊維 / negative regulation of gene expression / neuronal cell body / DNA damage response / 樹状突起 / protein kinase binding / enzyme binding / ミトコンドリア / DNA binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.851 Å | ||||||
データ登録者 | Eisenberg, D.S. / Boyer, D.R. | ||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: Biorxiv / 年: 2021 タイトル: A structure-based model for the electrostatic interaction of the N-terminus of protein tau with the fibril core of Alzheimer's Disease filaments 著者: Boyer, D.R. / Eisenberg, D.S. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6n4p.cif.gz | 11.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6n4p.ent.gz | 5.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6n4p.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n4/6n4p ftp://data.pdbj.org/pub/pdb/validation_reports/n4/6n4p | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: タンパク質・ペプチド | 分子量: 807.870 Da / 分子数: 2 / 断片: UNP residues 5-10 / 由来タイプ: 合成 / 由来: (合成) Homo sapiens (ヒト) / 参照: UniProt: P10636 #2: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶化 | 温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 / 詳細: 0.2 M ammonium citrate dibasic, 30% PEG3350 |
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-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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放射光源 | 由来: シンクロトロン / サイト: APS / ビームライン: 24-ID-E / 波長: 0.97 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
検出器 | タイプ: DECTRIS EIGER X 16M / 検出器: PIXEL / 日付: 2017年7月18日 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
放射波長 | 波長: 0.97 Å / 相対比: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
反射 | 解像度: 1.85→24.639 Å / Num. obs: 843 / % possible obs: 97.8 % / 冗長度: 2.868 % / Biso Wilson estimate: 14.78 Å2 / CC1/2: 0.971 / Rmerge(I) obs: 0.16 / Rrim(I) all: 0.196 / Χ2: 0.92 / Net I/σ(I): 4.16 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
反射 シェル | Diffraction-ID: 1
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-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 6-residue idealized beta sheet 解像度: 1.851→24.639 Å / SU ML: 0.15 / 交差検証法: THROUGHOUT / σ(F): 1.39 / 位相誤差: 19.28
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å | ||||||||||||||||||||||||
原子変位パラメータ | Biso max: 54.33 Å2 / Biso mean: 21.9015 Å2 / Biso min: 10.28 Å2 | ||||||||||||||||||||||||
精密化ステップ | サイクル: final / 解像度: 1.851→24.639 Å
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LS精密化 シェル | 解像度: 1.8515→1.918 Å / Rfactor Rfree error: 0 / Total num. of bins used: 1 /
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