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- PDB-6lzw: W513A mutant of chitin-specific solute binding protein from Vibri... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6lzw | |||||||||
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Title | W513A mutant of chitin-specific solute binding protein from Vibrio harveyi co-crystalized with chitobiose. | |||||||||
![]() | Peptide ABC transporter, periplasmic peptide-binding protein | |||||||||
![]() | SUGAR BINDING PROTEIN / complex / chitin / periplasmic solute-binding protein / Vibrios | |||||||||
Function / homology | ![]() ATP-binding cassette (ABC) transporter complex / transmembrane transport / outer membrane-bounded periplasmic space Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Kitaoku, Y. / Ubonbal, P. / Tran, L.T. / Robinson, R.C. / Suginta, W. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A structural model for (GlcNAc) 2 translocation via a periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria. Authors: Kitaoku, Y. / Fukamizo, T. / Kumsaoad, S. / Ubonbal, P. / Robinson, R.C. / Suginta, W. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.4 KB | Display | ![]() |
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PDB format | ![]() | 92.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6lzqC ![]() 6lztC ![]() 6lzuC ![]() 6lzvC ![]() 7ebiC ![]() 7ebmC ![]() 5yqwS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Sugars , 2 types, 2 molecules A
#1: Protein | Mass: 60930.648 Da / Num. of mol.: 1 / Mutation: W513A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: VME_26970 / Production host: ![]() ![]() |
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#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
-Non-polymers , 5 types, 137 molecules ![](data/chem/img/EDO.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-EDO / |
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#4: Chemical | ChemComp-CA / |
#5: Chemical | ChemComp-CL / |
#6: Chemical | ChemComp-MG / |
#7: Water | ChemComp-HOH / |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.96 % |
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Crystal grow | Temperature: 293 K / Method: microbatch / pH: 6.5 Details: 1.63mM chitobiose, 0.06M Divalents (MgCl2, CaCl2), 0.1M Buffer System 1 (Imidazole, MES (acid)), pH 6.5, 30% v/v Precipitant Mix 2 (Ethylene glycol, PEG 8000) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Dec 8, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99984 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 42466 / % possible obs: 95 % / Redundancy: 2.9 % / Rmerge(I) obs: 0.105 / Rpim(I) all: 0.075 / Rrim(I) all: 0.13 / Rsym value: 0.229 / Net I/σ(I): 9.81 |
Reflection shell | Resolution: 1.9→1.93 Å / Rmerge(I) obs: 0.757 / Num. unique obs: 2023 / CC1/2: 0.446 / Rpim(I) all: 0.63 / Rrim(I) all: 0.991 / Rsym value: 1.198 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5yqw Resolution: 1.9→26.888 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 33.39 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→26.888 Å
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Refine LS restraints |
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LS refinement shell |
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