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Yorodumi- PDB-6lz4: Crystal structure of PMab-1 Fv-clasp fragment with its antigen peptide -
+Open data
-Basic information
Entry | Database: PDB / ID: 6lz4 | |||||||||
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Title | Crystal structure of PMab-1 Fv-clasp fragment with its antigen peptide | |||||||||
Components |
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Keywords | IMMUNE SYSTEM / antibody fragment / Fv-clasp | |||||||||
Function / homology | Function and homology information tube morphogenesis / regulation of myofibroblast contraction / lymphatic endothelial cell fate commitment / actin-mediated cell contraction / GPVI-mediated activation cascade / leading edge of lamellipodium / visceral serous pericardium development / regulation of substrate adhesion-dependent cell spreading / positive regulation of extracellular matrix disassembly / lymphangiogenesis ...tube morphogenesis / regulation of myofibroblast contraction / lymphatic endothelial cell fate commitment / actin-mediated cell contraction / GPVI-mediated activation cascade / leading edge of lamellipodium / visceral serous pericardium development / regulation of substrate adhesion-dependent cell spreading / positive regulation of extracellular matrix disassembly / lymphangiogenesis / regulation of lamellipodium morphogenesis / tetraspanin-enriched microdomain / positive regulation of platelet aggregation / positive regulation of platelet activation / filopodium membrane / positive regulation of cell motility / anchoring junction / wound healing, spreading of cells / microvillus membrane / lung alveolus development / prostaglandin metabolic process / regulation of G1/S transition of mitotic cell cycle / lamellipodium membrane / Rho protein signal transduction / lymph node development / regulation of cell adhesion / positive regulation of epithelial to mesenchymal transition / ruffle / filopodium / lung development / cell-cell adhesion / ruffle membrane / cell migration / lamellipodium / regulation of cell shape / cytoplasmic vesicle / basolateral plasma membrane / cell population proliferation / cell adhesion / positive regulation of cell migration / membrane raft / apical plasma membrane / negative regulation of cell population proliferation / external side of plasma membrane / signaling receptor binding / negative regulation of apoptotic process / signal transduction / plasma membrane / cytosol Similarity search - Function | |||||||||
Biological species | Rattus norvegicus (Norway rat) Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.49 Å | |||||||||
Authors | Arimori, T. / Takagi, J. | |||||||||
Funding support | Japan, 2items
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Citation | Journal: J.Biochem. / Year: 2020 Title: Site-specific epitope insertion into recombinant proteins using the MAP tag system. Authors: Wakasa, A. / Kaneko, M.K. / Kato, Y. / Takagi, J. / Arimori, T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6lz4.cif.gz | 323.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6lz4.ent.gz | 222.8 KB | Display | PDB format |
PDBx/mmJSON format | 6lz4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6lz4_validation.pdf.gz | 465.4 KB | Display | wwPDB validaton report |
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Full document | 6lz4_full_validation.pdf.gz | 475.5 KB | Display | |
Data in XML | 6lz4_validation.xml.gz | 24.6 KB | Display | |
Data in CIF | 6lz4_validation.cif.gz | 33.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lz/6lz4 ftp://data.pdbj.org/pub/pdb/validation_reports/lz/6lz4 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Antibody | Mass: 19156.553 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The VH domain region (residues 1-113) of this chimeric protein is derived from Rattus norvegicus, while the SARAH domain region (residues 114-164) is derived from Homo sapiens. Source: (gene. exp.) Rattus norvegicus (Norway rat) / Production host: Escherichia coli (E. coli) #2: Antibody | Mass: 18424.848 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The VL domain region (residues 1-108) of this chimeric protein is derived from Rattus norvegicus, while the SARAH domain region (residues 109-159) is derived from Homo sapiens. Source: (gene. exp.) Rattus norvegicus (Norway rat) / Production host: Escherichia coli (E. coli) #3: Protein/peptide | Mass: 1429.615 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Mus musculus (house mouse) / References: UniProt: Q62011*PLUS Has protein modification | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.34 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M Potassium formate, 20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 27, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→48.15 Å / Num. obs: 23798 / % possible obs: 99.3 % / Redundancy: 8.7 % / Biso Wilson estimate: 61.08 Å2 / CC1/2: 0.998 / Rsym value: 0.13 / Net I/σ(I): 11.55 |
Reflection shell | Resolution: 2.49→2.64 Å / Num. unique obs: 3645 / CC1/2: 0.65 / Rsym value: 1.42 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1LK3, 5W5Z, 5XCT Resolution: 2.49→42.54 Å / SU ML: 0.4411 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 32.0949 / Stereochemistry target values: CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 79.66 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.49→42.54 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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