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Open data
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Basic information
Entry | Database: PDB / ID: 6lkk | ||||||
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Title | Two-component system protein mediate signal transduction | ||||||
![]() | ABC transporter, solute-binding protein | ||||||
![]() | SIGNALING PROTEIN / two-component system / G6P sensor / signal transduction | ||||||
Function / homology | thiamine binding / Bacterial extracellular solute-binding protein / thiamine transport / thiamine pyrophosphate binding / Prokaryotic membrane lipoprotein lipid attachment site profile. / outer membrane-bounded periplasmic space / 6-O-phosphono-alpha-D-glucopyranose / ABC transporter, solute-binding protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, M. / Tao, Y. | ||||||
![]() | ![]() Title: Interface switch mediates signal transmission in a two-component system. Authors: Wang, M. / Guo, Q. / Zhu, K. / Fang, B. / Yang, Y. / Teng, M. / Li, X. / Tao, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 132 KB | Display | ![]() |
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PDB format | ![]() | 102.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 771.5 KB | Display | ![]() |
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Full document | ![]() | 771.5 KB | Display | |
Data in XML | ![]() | 15.4 KB | Display | |
Data in CIF | ![]() | 23.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 33754.316 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: E4U00_07700, EP54_06205, EQ90_12025, HMPREF3211_02751, NCTC10654_00249, NCTC10702_00414, RK64_01575 Production host: ![]() ![]() |
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#2: Sugar | ChemComp-G6P / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 45.97 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: 0.1M Tris pH 7.5-7.9, 21% PEG 3350 / PH range: 7.5-7.9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 5, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 47810 / % possible obs: 99.9 % / Redundancy: 7.1 % / CC1/2: 0.997 / Rmerge(I) obs: 0.065 / Rpim(I) all: 0.021 / Rrim(I) all: 0.074 / Net I/σ(I): 15.36 |
Reflection shell | Resolution: 1.5→1.56 Å / Redundancy: 6.7 % / Rmerge(I) obs: 0.451 / Mean I/σ(I) obs: 2.42 / Num. unique obs: 3920 / CC1/2: 0.986 / Rpim(I) all: 0.135 / Rrim(I) all: 0.497 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 84.91 Å2 / Biso mean: 28.3628 Å2 / Biso min: 13.99 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.502→37.307 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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