+Open data
-Basic information
Entry | Database: PDB / ID: 6l7k | ||||||
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Title | solution structure of hIFABP V60C/Y70C variant. | ||||||
Components | Fatty acid-binding protein, intestinal | ||||||
Keywords | TRANSPORT PROTEIN / fatty acid binding protein / mutant / disulfide | ||||||
Function / homology | Function and homology information intestinal lipid absorption / apical cortex / long-chain fatty acid transmembrane transporter activity / long-chain fatty acid binding / Triglyceride catabolism / microvillus / fatty acid transport / fatty acid metabolic process / fatty acid binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Fan, J. / Yang, D. | ||||||
Funding support | Singapore, 1items
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Citation | Journal: Biophys.J. / Year: 2020 Title: Ligand Entry into Fatty Acid Binding Protein via Local Unfolding Instead of Gap Widening. Authors: Xiao, T. / Lu, Y. / Fan, J.S. / Yang, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6l7k.cif.gz | 816.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6l7k.ent.gz | 684.8 KB | Display | PDB format |
PDBx/mmJSON format | 6l7k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l7/6l7k ftp://data.pdbj.org/pub/pdb/validation_reports/l7/6l7k | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15042.022 Da / Num. of mol.: 1 / Mutation: V60C,Y70C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FABP2, FABPI / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: P12104 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 1 mM [U-99% 13C; U-99% 15N] human Intestinal fatty acid binding proteins (hIFABP), 90% H2O/10% D2O Label: 13C,15N_sample / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mM Component: human Intestinal fatty acid binding proteins (hIFABP) Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 20 mM / Ionic strength err: 0.1 / Label: condition_1 / pH: 7.0 / PH err: 0.05 / Pressure: 1 atm / Pressure err: 0.01 / Temperature: 298 K / Temperature err: 0.1 |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 3 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |