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Open data
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Basic information
Entry | Database: PDB / ID: 6l4v | |||||||||
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Title | Turning an asparaginyl endopeptidase into a peptide ligase | |||||||||
![]() | Asparaginyl endopeptidase | |||||||||
![]() | HYDROLASE / AEP / Asparaginyl Endopeptidase / Peptide asparaginyl Ligase / PAL | |||||||||
Function / homology | ![]() legumain / vacuolar protein processing / proteolysis involved in protein catabolic process / cysteine-type endopeptidase activity Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | El Sahili, A. / Lescar, J. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Turning an Asparaginyl Endopeptidase into a Peptide Ligase Authors: Hemu, X. / El Sahili, A. / Hu, S. / Zhang, X. / Serra, A. / Goh, B.C. / Darwis, D.A. / Chen, M.W. / Sze, S.K. / Liu, C.F. / Lescar, J. / Tam, J.P. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 112.8 KB | Display | ![]() |
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PDB format | ![]() | 82.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457.3 KB | Display | ![]() |
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Full document | ![]() | 460.7 KB | Display | |
Data in XML | ![]() | 22.8 KB | Display | |
Data in CIF | ![]() | 35.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6l4wC ![]() 6l4xC ![]() 6l4yC ![]() 6lkoC ![]() 5h0iS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 55773.875 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() References: UniProt: A0A0P0QM28, legumain | ||||||
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#2: Chemical | ChemComp-EDO / #3: Chemical | ChemComp-PEG / | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.38 % |
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Crystal grow | Temperature: 298 K / Method: evaporation / pH: 7.2 Details: 0.1M Sodium formate; 0.1M Ammonium acetate; 0.1M Sodium citrate tribasic dihydrate; 0.1M Potassium sodium tartrate tetrahydrate; 0.1M Sodium oxamate 0.1M Sodium HEPES; 0.1M MOPS 10% v/v ...Details: 0.1M Sodium formate; 0.1M Ammonium acetate; 0.1M Sodium citrate tribasic dihydrate; 0.1M Potassium sodium tartrate tetrahydrate; 0.1M Sodium oxamate 0.1M Sodium HEPES; 0.1M MOPS 10% v/v Ethylene glycol; 8 % w/v PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Apr 28, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.00004 Å / Relative weight: 1 |
Reflection | Resolution: 1.35→19.75 Å / Num. obs: 103635 / % possible obs: 99.8 % / Redundancy: 6.2 % / Rmerge(I) obs: 0.05729 / Net I/σ(I): 14.16 |
Reflection shell | Resolution: 1.35→1.39 Å / Rmerge(I) obs: 1.58 / Mean I/σ(I) obs: 0.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5H0I Resolution: 1.35→19.75 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.962 / SU R Cruickshank DPI: 0.049 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.053 / SU Rfree Blow DPI: 0.052 / SU Rfree Cruickshank DPI: 0.049
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Displacement parameters | Biso mean: 26.23 Å2
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Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.35→19.75 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.35→1.39 Å
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