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- PDB-6kie: Crystal structure of human leucyl-tRNA synthetase, Leu-AMS-bound form -
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Open data
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Basic information
Entry | Database: PDB / ID: 6kie | ||||||
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Title | Crystal structure of human leucyl-tRNA synthetase, Leu-AMS-bound form | ||||||
![]() | Leucine--tRNA ligase, cytoplasmic | ||||||
![]() | LIGASE / leucyl-tRNA synthetase / Leu-AMS-bound form | ||||||
Function / homology | ![]() glutamine-tRNA ligase activity / glutaminyl-tRNA aminoacylation / Selenoamino acid metabolism / leucine-tRNA ligase / leucine-tRNA ligase activity / leucyl-tRNA aminoacylation / cellular response to leucine starvation / Cytosolic tRNA aminoacylation / cellular response to L-leucine / tRNA aminoacylation for protein translation ...glutamine-tRNA ligase activity / glutaminyl-tRNA aminoacylation / Selenoamino acid metabolism / leucine-tRNA ligase / leucine-tRNA ligase activity / leucyl-tRNA aminoacylation / cellular response to leucine starvation / Cytosolic tRNA aminoacylation / cellular response to L-leucine / tRNA aminoacylation for protein translation / aminoacyl-tRNA editing activity / aminoacyl-tRNA synthetase multienzyme complex / regulation of cell size / positive regulation of TOR signaling / endomembrane system / positive regulation of TORC1 signaling / cellular response to amino acid starvation / GTPase activator activity / positive regulation of GTPase activity / cellular response to amino acid stimulus / lysosome / nuclear body / endoplasmic reticulum / ATP binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, S. / Son, J. / Kim, S. / Hwang, K.Y. | ||||||
![]() | ![]() Title: Leucine-sensing mechanism of leucyl-tRNA synthetase 1 for mTORC1 activation. Authors: Kim, S. / Yoon, I. / Son, J. / Park, J. / Kim, K. / Lee, J.H. / Park, S.Y. / Kang, B.S. / Han, J.M. / Hwang, K.Y. / Kim, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 223.2 KB | Display | ![]() |
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PDB format | ![]() | 173.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 756.9 KB | Display | ![]() |
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Full document | ![]() | 790.7 KB | Display | |
Data in XML | ![]() | 26.8 KB | Display | |
Data in CIF | ![]() | 39.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6kidC ![]() 6kqyC ![]() 6kr7C ![]() 1wz2S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 123003.312 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||||
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#2: Chemical | #3: Chemical | ChemComp-LSS / | #4: Chemical | ChemComp-LEU / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.63 Å3/Da / Density % sol: 78.89 % |
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Crystal grow | Temperature: 273 K / Method: vapor diffusion, hanging drop Details: 0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24% PEG3350 |
-Data collection
Diffraction | Mean temperature: 293 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jun 22, 2017 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.1→50 Å / Num. obs: 46132 / % possible obs: 96.4 % / Redundancy: 6.1 % / Rmerge(I) obs: 0.11 / Rpim(I) all: 0.043 / Rrim(I) all: 0.119 / Χ2: 2.59 / Net I/σ(I): 10.4 / Num. measured all: 280543 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 1wz2 Resolution: 3.15→46.848 Å / SU ML: 0.64 / Cross valid method: THROUGHOUT / σ(F): 1.49 / Phase error: 29.18 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 292.13 Å2 / Biso mean: 96.6088 Å2 / Biso min: 32.01 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.15→46.848 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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