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Open data
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Basic information
Entry | Database: PDB / ID: 6kcp | |||||||||
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Title | Shuguo PWWP in complex with ssDNA | |||||||||
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![]() | DNA BINDING PROTEIN/DNA / Shuguo / PWWP / histone reader / DNA binding / DNA BINDING PROTEIN-DNA complex | |||||||||
Function / homology | ![]() Formation of WDR5-containing histone-modifying complexes / chromatin remodeling / nucleus Similarity search - Function | |||||||||
Biological species | ![]() ![]() synthetic construct (others) | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Liu, Y.C. / Huang, Y. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Shuguo PWWP in complex with ssDNA Authors: Liu, Y.C. / Huang, Y. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.7 KB | Display | ![]() |
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PDB format | ![]() | 68.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6kcoC ![]() 6kd6S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9658.010 Da / Num. of mol.: 4 / Fragment: PWWP domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: DNA chain | | Mass: 1476.007 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.87 Å3/Da / Density % sol: 34.38 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / Details: 0.1M Tris-HCl, pH 8.5, 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jun 28, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97776 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→30 Å / Num. obs: 27752 / % possible obs: 99.9 % / Redundancy: 6.8 % / Rmerge(I) obs: 0.113 / Net I/σ(I): 17.8 |
Reflection shell | Resolution: 1.8→1.86 Å / Rmerge(I) obs: 0.291 / Num. unique obs: 2779 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6KD6 Resolution: 1.8→26.162 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 22.85
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→26.162 Å
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Refine LS restraints |
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LS refinement shell |
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