Mass: 22161.982 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: APOBEC3G / Production host: Escherichia coli (E. coli) References: UniProt: Q9HC16, Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In cyclic amidines
#2: DNA chain
DNA (5'-D(*AP*TP*TP*CP*UP*(IUR)P*AP*AP*TP*T)-3')
Mass: 3106.845 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
3
anisotropic
1
3D HNCO
1
2
3
anisotropic
1
3D HNCA
1
3
3
anisotropic
1
3DHN(CO)CA
1
4
3
anisotropic
1
3D HN(CA)CB
1
5
1
anisotropic
1
2D 1H-15N HSQC
1
6
2
anisotropic
4
3D 1H-13C NOESY aliphatic
1
7
2
anisotropic
4
3D 1H-13C NOESY aromatic
1
8
1
anisotropic
2
3D 1H-15N NOESY
1
9
2
anisotropic
4
3D (H)CCH-TOCSY
1
14
1
anisotropic
2
3D 1H-15N TOCSY
1
10
2
anisotropic
4
2D 1H-13C HSQC aliphatic
1
11
2
anisotropic
4
2D 1H-13C HSQC aromatic
1
12
4
anisotropic
3
2D DQF-COSY
1
13
4
anisotropic
3
2D 1H-1H TOCSY
1
15
4
anisotropic
3
2D 1H-1H NOESY
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Sample preparation
Details
Type
Solution-ID
Contents
Label
Solvent system
solution
1
0.35 mM [U-99% 13C; U-99% 15N] DNA dC->dU-editing enzyme APOBEC3G, 0.525 mM DNA (5'-D(*AP*TP*TP*CP*UP*(IUR)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 5 mM DTT, 50 uM ZINC ION, 90% H2O/10% D2O
13C,15N_sample
90% H2O/10% D2O
solution
2
0.35 mM [U-99% 13C; U-99% 15N] DNA dC->dU-editing enzyme APOBEC3G, 0.525 mM DNA (5'-D(*AP*TP*TP*CP*UP*(IUR)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 5 mM DTT, 50 uM ZINC ION, 100% D2O
13C,15N_sample_2
100% D2O
solution
3
0.35 mM [U-100% 13C; U-100% 15N; U-80% 2H] DNA dC->dU-editing enzyme APOBEC3G, 0.525 mM DNA (5'-D(*AP*TP*TP*CP*UP*(IUR)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 5 mM DTT, 50 uM ZINC ION, 90% H2O/10% D2O
13C,15N,2H_sample
90% H2O/10% D2O
solution
4
1 mM DNA (5'-D(*AP*TP*TP*CP*UP*(IUR)P*AP*AP*TP*T)-3'), 50 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O