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Open data
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Basic information
Entry | Database: PDB / ID: 6jxk | ||||||
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Title | Rb+-bound E2-MgF state of the gastric proton pump (Wild-type) | ||||||
![]() | (Potassium-transporting ATPase ...) x 2 | ||||||
![]() | MEMBRANE PROTEIN / P-type ATPase / proton pump / gastric / ion pump | ||||||
Function / homology | ![]() H+/K+-exchanging ATPase / potassium:proton exchanging ATPase complex / P-type potassium:proton transporter activity / Ion transport by P-type ATPases / P-type sodium:potassium-exchanging transporter activity / sodium:potassium-exchanging ATPase complex / sodium ion export across plasma membrane / intracellular potassium ion homeostasis / intracellular sodium ion homeostasis / potassium ion import across plasma membrane ...H+/K+-exchanging ATPase / potassium:proton exchanging ATPase complex / P-type potassium:proton transporter activity / Ion transport by P-type ATPases / P-type sodium:potassium-exchanging transporter activity / sodium:potassium-exchanging ATPase complex / sodium ion export across plasma membrane / intracellular potassium ion homeostasis / intracellular sodium ion homeostasis / potassium ion import across plasma membrane / potassium ion binding / ATPase activator activity / potassium ion transmembrane transport / proton transmembrane transport / cell adhesion / apical plasma membrane / magnesium ion binding / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Abe, K. / Irie, K. / Yamamoto, K. | ||||||
![]() | ![]() Title: A single K + -binding site in the crystal structure of the gastric proton pump. Authors: Yamamoto, K. / Dubey, V. / Irie, K. / Nakanishi, H. / Khandelia, H. / Fujiyoshi, Y. / Abe, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1 MB | Display | ![]() |
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PDB format | ![]() | 858.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 795.8 KB | Display | ![]() |
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Full document | ![]() | 835 KB | Display | |
Data in XML | ![]() | 88.6 KB | Display | |
Data in CIF | ![]() | 117 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6jxhC ![]() 6jxiC ![]() 6jxjC ![]() 5yluS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Potassium-transporting ATPase ... , 2 types, 4 molecules AEBF
#1: Protein | Mass: 109197.672 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 32982.652 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 1 types, 8 molecules ![](data/chem/img/NAG.gif)
#7: Sugar | ChemComp-NAG / |
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-Non-polymers , 4 types, 12 molecules ![](data/chem/img/MF4.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/CLR.gif)
![](data/chem/img/K.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/CLR.gif)
![](data/chem/img/K.gif)
#3: Chemical | #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-K / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.36 Å3/Da / Density % sol: 71.78 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 10% glycerol, 15% PEG6000, 0.1M CH3COOK, 6% methylpentanediol, 5 mM beta-mercaptoethanol |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 14, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 4.3→48 Å / Num. obs: 31390 / % possible obs: 94.94 % / Redundancy: 6.6 % / Biso Wilson estimate: 158.44 Å2 / CC1/2: 0.99 / Net I/σ(I): 7.19 |
Reflection shell | Resolution: 4.3→4.5 Å / Mean I/σ(I) obs: 0.92 / CC1/2: 0.75 / % possible all: 53.46 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5ylu Resolution: 4.3→47.79 Å / SU ML: 0.7173 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 39.8419
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 244.02 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.3→47.79 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 217.7477 Å / Origin y: -32.2571 Å / Origin z: 56.926 Å
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Refinement TLS group |
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