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Open data
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Basic information
Entry | Database: PDB / ID: 6jh7 | ||||||
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Title | Crystal structure of AerF from Microcystis aeruginosa | ||||||
![]() | Short chain dehydrogenase family protein | ||||||
![]() | BIOSYNTHETIC PROTEIN / reductase | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Qiu, X. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional investigation of AerF, a NADPH-dependent alkenal double bond reductase participating in the biosynthesis of Choi moiety of aeruginosin Authors: Qiu, X. / Wei, Y. / Zhu, W. / Fu, J. / Duan, X. / Jin, H. / Zhu, P. / Zhou, C. / Yan, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 241.3 KB | Display | ![]() |
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PDB format | ![]() | 194.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 477.2 KB | Display | ![]() |
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Full document | ![]() | 488.8 KB | Display | |
Data in XML | ![]() | 27.5 KB | Display | |
Data in CIF | ![]() | 40.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6jhaC ![]() 6jhbC ![]() 3u49S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 30341.568 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: aerF, C789_498 / Production host: ![]() ![]() #2: Chemical | ChemComp-PGE / | #3: Chemical | ChemComp-ACT / | #4: Chemical | ChemComp-PEG / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.15 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, sitting drop Details: 0.2 M Calcium Chloride, 0.1 M HEPES sodium pH 7.0, 23% (v/v) polyethylene glycol 400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 15, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97907 Å / Relative weight: 1 |
Reflection | Resolution: 1.38→45 Å / Num. obs: 122470 / % possible obs: 98.5 % / Redundancy: 13.7 % / Rmerge(I) obs: 0.083 / Rpim(I) all: 0.023 / Net I/σ(I): 33.9 |
Reflection shell | Resolution: 1.38→1.4 Å / Redundancy: 9.4 % / Rmerge(I) obs: 0.906 / Mean I/σ(I) obs: 3.3 / Num. unique obs: 5628 / Rpim(I) all: 0.301 / % possible all: 92.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3U49 Resolution: 1.38→45 Å / Cor.coef. Fo:Fc: 0.972 / Cor.coef. Fo:Fc free: 0.961 / SU B: 1.335 / SU ML: 0.025 / Cross valid method: THROUGHOUT / ESU R: 0.05 / ESU R Free: 0.047 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.757 Å2
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Refinement step | Cycle: 1 / Resolution: 1.38→45 Å
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Refine LS restraints |
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