+Open data
-Basic information
Entry | Database: PDB / ID: 6jbr | ||||||||||||
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Title | Tps1/UDP/T6P complex | ||||||||||||
Components | Trehalose-6-phosphate synthase | ||||||||||||
Keywords | TRANSFERASE / Trehalose-6-phosphate synthase | ||||||||||||
Function / homology | Function and homology information alpha,alpha-trehalose-phosphate synthase (UDP-forming) / alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity / alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) / trehalose-phosphatase activity / ascospore formation / trehalose biosynthetic process / trehalose metabolism in response to stress / cellular response to heat / nucleotide binding / cytosol Similarity search - Function | ||||||||||||
Biological species | Pyricularia oryzae 70-15 (fungus) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.03 Å | ||||||||||||
Authors | Wang, S. / Zhao, Y. / Wang, D. / Liu, J. | ||||||||||||
Funding support | China, 1items
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Citation | Journal: Biochem.J. / Year: 2019 Title: Crystal structures of Magnaporthe oryzae trehalose-6-phosphate synthase (MoTps1) suggest a model for catalytic process of Tps1. Authors: Wang, S. / Zhao, Y. / Yi, L. / Shen, M. / Wang, C. / Zhang, X. / Yang, J. / Peng, Y.L. / Wang, D. / Liu, J. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6jbr.cif.gz | 778.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6jbr.ent.gz | 636.3 KB | Display | PDB format |
PDBx/mmJSON format | 6jbr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6jbr_validation.pdf.gz | 6 MB | Display | wwPDB validaton report |
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Full document | 6jbr_full_validation.pdf.gz | 6.1 MB | Display | |
Data in XML | 6jbr_validation.xml.gz | 153.2 KB | Display | |
Data in CIF | 6jbr_validation.cif.gz | 211.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jb/6jbr ftp://data.pdbj.org/pub/pdb/validation_reports/jb/6jbr | HTTPS FTP |
-Related structure data
Related structure data | 6jakC 6jbiC 6jbwC 1uqtS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 52512.719 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyricularia oryzae 70-15 (fungus) / Strain: 70-15 / ATCC MYA-4617 / FGSC 8958 / Gene: MGG_03860 / Production host: Escherichia coli (E. coli) References: UniProt: G4NHF4, alpha,alpha-trehalose-phosphate synthase (UDP-forming) #2: Polysaccharide | 6-O-phosphono-alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose / trehalose-6-phosphate #3: Chemical | ChemComp-UDP / #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 57.04 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 8.9 Details: 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, 20% PEG 3350 (v/v) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9785 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 23, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→30 Å / Num. obs: 305744 / % possible obs: 99.6 % / Redundancy: 3.5 % / CC1/2: 1 / Rmerge(I) obs: 0.057 / Rpim(I) all: 0.023 / Net I/σ(I): 32.26 |
Reflection shell | Resolution: 2.03→2.1 Å / Rmerge(I) obs: 0.451 / Num. unique obs: 30304 / CC1/2: 0.892 / Rpim(I) all: 0.195 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1UQT Resolution: 2.03→29.798 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 2.03→29.798 Å
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LS refinement shell | Resolution: 2.03→2.05 Å
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