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Open data
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Basic information
Entry | Database: PDB / ID: 6j6c | ||||||
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Title | Borrelia burgdorferi OspA via surface entropy reduction (form3) | ||||||
![]() | Outer surface protein A | ||||||
![]() | LIPID BINDING PROTEIN / single-layer beta-sheet | ||||||
Function / homology | Outer surface lipoprotein, Borrelia / Outer surface lipoprotein domain superfamily / Borrelia lipoprotein / cell outer membrane / Prokaryotic membrane lipoprotein lipid attachment site profile. / cell surface / membrane / Outer surface protein A![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fujiwara, H. / Makabe, K. | ||||||
![]() | ![]() Title: Beta-sheet elasticity of peptide self-assembly mimic, PSAM, with a grafted sequence characterized by comprehensive analyses of isomorphous crystals Authors: Fujiwara, H. / Hongo, K. / Hori, Y. / Yoshida, N. / Makabe, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66 KB | Display | ![]() |
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PDB format | ![]() | 45.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.5 KB | Display | ![]() |
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Full document | ![]() | 418.6 KB | Display | |
Data in XML | ![]() | 12.8 KB | Display | |
Data in CIF | ![]() | 19 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5b2aC ![]() 6j5mC ![]() 6j5nC ![]() 6j5oC ![]() 6j5pC ![]() 6j5qC ![]() 6j5rC ![]() 6j6bC ![]() 6j6dC ![]() 6j6eC ![]() 2g8cS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26395.469 Da / Num. of mol.: 1 Mutation: E37S,E45S,K46S,K48A,K60A,K64S,K83A,E104S,K107S,E196A,K239S,E240S,K254S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680 / Gene: ospA, BB_A15 / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.32 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 0.1 M Imidazole pH 7.0, 45% PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Dec 2, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20 Å / Num. obs: 30494 / % possible obs: 99.8 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.076 / Net I/σ(I): 23.4 |
Reflection shell | Resolution: 1.6→1.63 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.422 / Mean I/σ(I) obs: 1.92 / Num. unique obs: 1490 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2G8C Resolution: 1.6→19.715 Å / SU ML: 0.16 / Cross valid method: THROUGHOUT / σ(F): 1.38 / Phase error: 21.28
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→19.715 Å
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Refine LS restraints |
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LS refinement shell |
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