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- PDB-6j47: OspA variant with a short chameleon sequence from alpha B crystallin -
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Open data
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Basic information
Entry | Database: PDB / ID: 6j47 | |||||||||
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Title | OspA variant with a short chameleon sequence from alpha B crystallin | |||||||||
![]() | Outer surface protein A | |||||||||
![]() | LIPID BINDING PROTEIN / beta-sheet / DE NOVO PROTEIN | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Makabe, K. / Hori, Y. | |||||||||
![]() | ![]() Title: Grafting a short chameleon sequence from alpha B crystallin into a beta-sheet scaffold protein. Authors: Hori, Y. / Fujiwara, H. / Fujiwara, W. / Makabe, K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.2 KB | Display | ![]() |
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PDB format | ![]() | 43.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 455.3 KB | Display | ![]() |
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Full document | ![]() | 458.4 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 15.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6j48C ![]() 6j49C ![]() 2g8cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26345.543 Da / Num. of mol.: 1 Mutation: E37S, E45S, K46S,K64S, E104S, K107S, K239S, E240S, K254S, K48A, K60A, K83A, E196A, S120V,S121L, T122G, E123D, E124V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ospA / Production host: ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-TRS / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 42.02 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 9 Details: 40% PEG 400, 0.1M Tris-HCl pH 9.0, protein 10 mg/mL |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 29, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→63.32 Å / Num. obs: 17284 / % possible obs: 98.3 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.075 / Net I/σ(I): 24 |
Reflection shell | Resolution: 1.9→1.93 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 4.72 / Num. unique obs: 833 / % possible all: 96.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2G8C Resolution: 1.9→27.002 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 31.81
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→27.002 Å
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Refine LS restraints |
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LS refinement shell |
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