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- PDB-6j5n: OspA mutant, PSAM-VLGDV1-form4, grafted short chameleon sequence ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6j5n | ||||||
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Title | OspA mutant, PSAM-VLGDV1-form4, grafted short chameleon sequence from alpha-B crystallin | ||||||
![]() | Outer surface protein A | ||||||
![]() | LIPID BINDING PROTEIN / Peptide Self-Assembly Mimic / single-layer beta-sheet / chameleon sequence | ||||||
Function / homology | Outer surface lipoprotein, Borrelia / Outer surface lipoprotein domain superfamily / Borrelia lipoprotein / cell outer membrane / Prokaryotic membrane lipoprotein lipid attachment site profile. / cell surface / membrane / Outer surface protein A / Outer surface protein A![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fujiwara, H. / Makabe, K. | ||||||
![]() | ![]() Title: Beta-sheet elasticity of peptide self-assembly mimic, PSAM, with a grafted sequence characterized by comprehensive analyses of isomorphous crystals Authors: Fujiwara, H. / Hongo, K. / Hori, Y. / Yoshida, N. / Makabe, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.8 KB | Display | ![]() |
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PDB format | ![]() | 46.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5b2aSC ![]() 6j5mC ![]() 6j5oC ![]() 6j5pC ![]() 6j5qC ![]() 6j5rC ![]() 6j6bC ![]() 6j6cC ![]() 6j6dC ![]() 6j6eC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26345.543 Da / Num. of mol.: 1 Mutation: E37S, E45S, K46S,K64S, E104S, K107S, K239S, E240S, K254S, K48A, K60A, K83A, E196A, S120V,S121L, T122G, E123D, E124V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ospA / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.55 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 0.1 M Tris-HCl pH 8.0, 38% PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Jun 11, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.73→20 Å / Num. obs: 24241 / % possible obs: 99.6 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 26.2 |
Reflection shell | Resolution: 1.73→1.76 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.549 / Mean I/σ(I) obs: 2.96 / Num. unique obs: 1226 / % possible all: 99.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5B2A Resolution: 1.73→19.826 Å / SU ML: 0.17 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 23.55
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.73→19.826 Å
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Refine LS restraints |
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LS refinement shell |
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