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- PDB-6iuq: Crystal structure and expression patterns of prolyl 4-hydroxylase... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6iuq | ||||||
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Title | Crystal structure and expression patterns of prolyl 4-hydroxylases from Phytophthora capsici | ||||||
![]() | Prolyl 4-hydroxylase | ||||||
![]() | OXIDOREDUCTASE / hydroxylase / iron / DSBH core fold | ||||||
Function / homology | ![]() L-ascorbic acid binding / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen / dioxygenase activity / iron ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Song, W.W. / Zhang, X.G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure and expression patterns of prolyl 4-hydroxylases from Phytophthora capsici Authors: Song, W.W. / Zhang, X.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 135.2 KB | Display | ![]() |
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PDB format | ![]() | 110.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.6 KB | Display | ![]() |
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Full document | ![]() | 443 KB | Display | |
Data in XML | ![]() | 24.9 KB | Display | |
Data in CIF | ![]() | 35.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40481.461 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: each monomer consisits of an iron ion Source: (gene. exp.) ![]() Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | GenBank number of this polypeptide is MH251252. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.04 Å3/Da / Density % sol: 65.31 % |
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Crystal grow | Temperature: 283 K / Method: vapor diffusion, sitting drop Details: 0.1M sodium citrate tribasic dehydrate pH5.0, 18% (w/v) PEG20000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 22, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.348→50 Å / Num. obs: 38776 / % possible obs: 98 % / Redundancy: 5.3 % / Rpim(I) all: 0.029 / Net I/σ(I): 8.7 |
Reflection shell | Resolution: 2.35→2.39 Å / Num. unique obs: 1882 / Rpim(I) all: 0.172 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.348→43.669 Å
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Refine LS restraints |
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LS refinement shell |
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