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- PDB-6ipv: Crystal structure of CqsB2 from Streptomyces exfoliatus 2419-SVT2... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ipv | ||||||
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Title | Crystal structure of CqsB2 from Streptomyces exfoliatus 2419-SVT2 (apo form) | ||||||
![]() | CqsB2 | ||||||
![]() | BIOSYNTHETIC PROTEIN / Carquinostatin A biosynthesis / polyketide aromatase/cyclase | ||||||
Function / homology | START-like domain superfamily / SRPBCC family protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tomita, T. / Kobayashi, M. / Nishiyama, M. / Kuzuyama, T. | ||||||
![]() | ![]() Title: An Unprecedented Cyclization Mechanism in the Biosynthesis of Carbazole Alkaloids in Streptomyces. Authors: Kobayashi, M. / Tomita, T. / Shin-Ya, K. / Nishiyama, M. / Kuzuyama, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 191.1 KB | Display | ![]() |
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PDB format | ![]() | 155.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.5 KB | Display | ![]() |
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Full document | ![]() | 452.3 KB | Display | |
Data in XML | ![]() | 35.1 KB | Display | |
Data in CIF | ![]() | 50.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27645.105 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Sequence details | The sequence has been deposited to DDBJ with accession code LC406090. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.92 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.1M Tris-HCl (pH8.5), 30% (w/v) PEG 4000, 0.2M LiSO4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Nov 25, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→125.72 Å / Num. obs: 53154 / % possible obs: 100 % / Redundancy: 14.7 % / CC1/2: 0.991 / Rmerge(I) obs: 0.128 / Rpim(I) all: 0.054 / Net I/σ(I): 21.9 |
Reflection shell | Resolution: 2.2→2.24 Å / Rmerge(I) obs: 0.278 / Num. unique obs: 2605 / CC1/2: 0.879 / Rpim(I) all: 0.266 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.843 Å2
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Refinement step | Cycle: 1 / Resolution: 2.2→125.72 Å
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Refine LS restraints |
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