D-threo-aldose 1-dehydrogenase activity / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor Similarity search - Function
Mass: 37285.402 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Clostridium beijerinckii (bacteria) / Gene: gpr_2, CBEIJ_11710 / Plasmid: pET-14b / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: A0A1S8QGT2, Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor
Mass: 18.015 Da / Num. of mol.: 264 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.18 Å3/Da / Density % sol: 61.34 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 90 mM MOPS pH 7.6, 271 mM NH4Cl2, 2.7% PEG 8K, 10 mM NADPH, 2 mM 4-pyridine methanol
Resolution: 1.75→54.58 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.952 / SU B: 4.428 / SU ML: 0.07 / Cross valid method: THROUGHOUT / ESU R: 0.092 / ESU R Free: 0.091 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.19274
2105
4.9 %
RANDOM
Rwork
0.16471
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obs
0.16605
40627
99.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å