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Open data
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Basic information
Entry | Database: PDB / ID: 6hbi | ||||||
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Title | SCAPHARCA DIMERIC HEMOGLOBIN, MUTANT T72V, DEOXY FORM | ||||||
![]() | HEMOGLOBIN | ||||||
![]() | OXYGEN TRANSPORT / HEME / RESPIRATORY PROTEIN | ||||||
Function / homology | ![]() oxygen carrier activity / oxygen binding / response to hypoxia / heme binding / identical protein binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Royer Junior, W.E. | ||||||
![]() | ![]() Title: Mutational destabilization of the critical interface water cluster in Scapharca dimeric hemoglobin: structural basis for altered allosteric activity. Authors: Pardanani, A. / Gambacurta, A. / Ascoli, F. / Royer Jr., W.E. #1: ![]() Title: Ordered Water Molecules as Key Allosteric Mediators in a Cooperative Dimeric Hemoglobin Authors: Royer Junior, W.E. / Pardanani, A. / Gibson, Q.H. / Peterson, E.S. / Friedman, J.M. #2: ![]() Title: High-Resolution Crystallographic Analysis of a Co-Operative Dimeric Hemoglobin Authors: Royer Junior, W.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.4 KB | Display | ![]() |
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PDB format | ![]() | 54.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4hbiC ![]() 5hbiC ![]() 7hbiC ![]() 4sdhS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.3511, -0.3203, -0.8798), Vector: |
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Components
#1: Protein | Mass: 15965.331 Da / Num. of mol.: 2 / Mutation: T72V Source method: isolated from a genetically manipulated source Details: SCAPHARCA DIMERIC HEMOGLOBIN, HEME GROUP, PROTOPORPHYRIN IX IRON Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 45 % | |||||||||||||||
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Crystal grow | pH: 8.5 Details: PROTEIN WAS CRYSTALLIZED FROM 2.1M NA/K PHOSPHATE AT PH 8.5 | |||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: batch method / Details: Royer Junior, W.E., (1994) J.Mol.Biol., 235, 657. | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Mar 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→20 Å / Num. obs: 24855 / % possible obs: 90.2 % / Observed criterion σ(I): 1 / Redundancy: 5.6 % / Rmerge(I) obs: 0.089 |
Reflection | *PLUS Num. measured all: 140113 |
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Processing
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Refinement | Method to determine structure: ISOMORPHOUS MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4SDH Resolution: 1.8→10 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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