温度: 300 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: Protein was concentrated to 25 mg/mL in [20 mM Tris (pH8.0), 250 mM NaCl]. Crystals of Reg576 were obtained in 130 mM potassium bromide, 25% PEG 2000 monomethylether by the sitting-drop vapor- ...詳細: Protein was concentrated to 25 mg/mL in [20 mM Tris (pH8.0), 250 mM NaCl]. Crystals of Reg576 were obtained in 130 mM potassium bromide, 25% PEG 2000 monomethylether by the sitting-drop vapor-diffusion method at 18 deg. C, in drops of 1 uL + 1 uL (protein:reservoir). Needle-shaped crystals appeared in less t han a week. Cryo-cooling in liquid nitrogen was carried out using a cryo-protecting solution containing reservoir solution supplemented with 5% glycerol
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データ収集
回折
平均測定温度: 100 K / Ambient temp details: Oxford cryostream 600 series
放射光源
由来: シンクロトロン / サイト: ALBA / ビームライン: XALOC / 波長: 0.9789 Å