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- PDB-6ghy: Structure of Lytic Transglycosylase MltE inactive mutant E64Q fro... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ghy | ||||||
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Title | Structure of Lytic Transglycosylase MltE inactive mutant E64Q from E.coli | ||||||
![]() | Endo-type membrane-bound lytic murein transglycosylase A | ||||||
![]() | LYASE / Lytic Transglycosylase | ||||||
Function / homology | ![]() : / lytic endotransglycosylase activity / lytic transglycosylase activity / peptidoglycan metabolic process / cell outer membrane / cell wall organization / cell wall macromolecule catabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Batuecas, M.T. / Hermoso, J.A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A Structural Dissection of the Active Site of the Lytic Transglycosylase MltE from Escherichia coli. Authors: Dik, D.A. / Batuecas, M.T. / Lee, M. / Mahasenan, K.V. / Marous, D.R. / Lastochkin, E. / Fisher, J.F. / Hermoso, J.A. / Mobashery, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.6 KB | Display | ![]() |
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PDB format | ![]() | 70.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.6 KB | Display | ![]() |
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Full document | ![]() | 445 KB | Display | |
Data in XML | ![]() | 19.4 KB | Display | |
Data in CIF | ![]() | 28.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ghzC ![]() 6gi3C ![]() 6gi4C ![]() 2y8pS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 21413.326 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: emtA, mltE, sltZ, ycgP, b1193, JW5821 / Production host: ![]() ![]() References: UniProt: P0C960, Lyases; Carbon-oxygen lyases; Acting on polysaccharides #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.02 % |
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Crystal grow | Temperature: 291 K / Method: batch mode / pH: 8.4 Details: 26% Polyethylene Glycol 4000, 0.2 M magnesium chloride and 0.1 M TRIS HCl (pH 8.4) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 12, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97242 Å / Relative weight: 1 |
Reflection | Resolution: 2.12→39.55 Å / Num. obs: 22063 / % possible obs: 98.06 % / Redundancy: 4.2 % / Rpim(I) all: 0.047 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.12→2.19 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2Y8P Resolution: 2.12→39.55 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.929 / SU B: 0.003 / SU ML: 0 / Cross valid method: THROUGHOUT / ESU R: 0.173 / ESU R Free: 0.209 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||
Displacement parameters | Biso mean: 35.334 Å2
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Refinement step | Cycle: 1 / Resolution: 2.12→39.55 Å
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LS refinement shell | Resolution: 2.12→2.175 Å / Total num. of bins used: 20
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