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Yorodumi- PDB-6g9g: Crystal Structure of the TASNSS segment from the R4-R5 loop of th... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6g9g | ||||||
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Title | Crystal Structure of the TASNSS segment from the R4-R5 loop of the E. coli Biofilm-associated CsgA Curli protein | ||||||
Components | Major curlin subunit | ||||||
Keywords | PROTEIN FIBRIL / Bacterial fibril from E. coli | ||||||
Function / homology | Curlin associated / Curlin associated repeat / regulation of amyloid fibril formation / single-species biofilm formation / pilus / amyloid fibril formation / cell adhesion / identical protein binding / Major curlin subunit Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.6 Å | ||||||
Model details | Curli | ||||||
Authors | Landau, M. / Perov, S. | ||||||
Citation | Journal: Plos Pathog. / Year: 2019 Title: Structural Insights into Curli CsgA Cross-beta Fibril Architecture Inspire Repurposing of Anti-amyloid Compounds as Anti-biofilm Agents. Authors: Perov, S. / Lidor, O. / Salinas, N. / Golan, N. / Tayeb-Fligelman, E. / Deshmukh, M. / Willbold, D. / Landau, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6g9g.cif.gz | 9.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6g9g.ent.gz | 4.3 KB | Display | PDB format |
PDBx/mmJSON format | 6g9g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g9/6g9g ftp://data.pdbj.org/pub/pdb/validation_reports/g9/6g9g | HTTPS FTP |
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-Related structure data
Related structure data | 6g8cC 6g8dC 6g8eC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 565.533 Da / Num. of mol.: 1 Fragment: Amyloid spine segment TASNSS from CsgA (residues 129-134) secreted by E. coli Source method: obtained synthetically / Details: TASNSS from CsgA, synthesized / Source: (synth.) Escherichia coli (E. coli) / References: UniProt: P28307 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: Reservoir contained 0.2 M Lithium sulfate, 0.1 M Tris-HCl pH 8.5, 30%(w/v) PEG 4000, and 10 mM of the TAIVVQ peptide |
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 0.9763 Å | ||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: May 2, 2016 | ||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.6→10.81 Å / Num. obs: 389 / % possible obs: 97.7 % / Redundancy: 8.686 % / Biso Wilson estimate: 18.334 Å2 / CC1/2: 0.986 / Rmerge(I) obs: 0.222 / Rrim(I) all: 0.237 / Χ2: 0.904 / Net I/σ(I): 6.07 / Num. measured all: 3379 | ||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: molecular replacement | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.6→10.81 Å / Cor.coef. Fo:Fc: 0.989 / Cor.coef. Fo:Fc free: 0.988 / SU B: 1.859 / SU ML: 0.059 / SU R Cruickshank DPI: 0.0803 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.08 / ESU R Free: 0.077 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 20.74 Å2 / Biso mean: 8.967 Å2 / Biso min: 7.9 Å2
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Refinement step | Cycle: final / Resolution: 1.6→10.81 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.603→1.787 Å / Rfactor Rfree error: 0 / Total num. of bins used: 5
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